Logo: to the web site of Uppsala University

uu.sePublikasjoner fra Uppsala universitet
Endre søk
RefereraExporteraLink to record
Permanent link

Direct link
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association
  • vancouver
  • Annet format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annet språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf
Quantitative overview of N2 fixation in Nostoc punctiforme ATCC 29133 through cellular enrichments and iTRAQ shotgun proteomics
Department of Chemical and Process Engineering, The University of Sheffield. (Biological & Environmental Systems Group)
Department of Chemical and Process Engineering, The University of Sheffield. (Biological & Environmental Systems Group)
Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Kemiska sektionen, Institutionen för fotokemi och molekylärvetenskap, Molekylär biomimetik.
Virginia Commonwealth University. (Center for the Study of Biological Complexity)
Vise andre og tillknytning
2009 (engelsk)Inngår i: Journal of Proteome Research, ISSN 1535-3893, E-ISSN 1535-3907, Vol. 8, nr 1, s. 187-198Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

Nostoc punctiforme ATCC 29133 is a photoautotrophic cyanobacterium with the capacity to fix atmospheric N2. Its ability to mediate this process is similar to that described for Nostoc sp. PCC 7120, where vegetative cells differentiate into heterocysts. Quantitative proteomic investigations at both the filament level and the heterocyst level are presented using isobaric tagging technology (iTRAQ), with 721 proteins at the 95% confidence interval quantified across both studies. Observations from both experiments yielded findings confirmatory of both transcriptional studies, and published Nostoc sp. PCC 7120 iTRAQ data. N. punctiforme exhibits similar metabolic trends, though changes in a number of metabolic pathways are less pronounced than in Nostoc sp. PCC 7120. Results also suggest a number of proteins that may benefit from future investigations. These include ATP dependent Zn-proteases, N-reserve degraders and also redox balance proteins. Complementary proteomic data sets from both organisms present key precursor knowledge that is important for future cyanobacterial biohydrogen research.

sted, utgiver, år, opplag, sider
USA: ACS Publications , 2009. Vol. 8, nr 1, s. 187-198
Emneord [en]
iTRAQ, Shotgun proteomics, Nostoc punctiforme ATCC 29133, Nostoc sp PCC 73102, Tandem-MS, Dinitrogen Fixation, Heterocysts
HSV kategori
Forskningsprogram
Biokemi
Identifikatorer
URN: urn:nbn:se:uu:diva-108462DOI: 10.1021/pr800285vISI: 000262171100021OAI: oai:DiVA.org:uu-108462DiVA, id: diva2:235948
Tilgjengelig fra: 2009-09-18 Laget: 2009-09-18 Sist oppdatert: 2017-12-13
Inngår i avhandling
1. The Heterocysts of Nostoc punctiforme: From Proteomics to Energy Transfer
Åpne denne publikasjonen i ny fane eller vindu >>The Heterocysts of Nostoc punctiforme: From Proteomics to Energy Transfer
2009 (engelsk)Doktoravhandling, med artikler (Annet vitenskapelig)
Abstract [en]

The aim of this thesis is to provide a thorough characterization of the photosynthetic machinery from the heterocysts of Nostoc punctiforme strain ATCC 29133. In this thesis I describe the protocols I have optimized for the isolation of thylakoids from vegetative cells, the purification of heterocysts and the isolation of thylakoids from the purified heterocysts. The composition of the thylakoid membranes was studied by two dimensional electrophoresis and mass-spectrometry. Further insight into the functionality of the photosynthetic complexes was obtained by EPR, electron transport measurements through Photosystem II (PSII), and fluorescence spectroscopy. The proteome of the heterocysts thylakoids compared to that of the vegetative cell was found to be dominated by Photosystem I (PSI) and ATP-synthase complexes, both essential for keeping high nitrogenase activities. Surprisingly, we found a significant amount of assembled monomeric PSII complexes in the heterocysts thylakoid membranes. We measured in vitro light-driven electron transfer from PSII in heterocysts using an artificial electron donor, suggesting that under certain circumstances heterocysts might activate PSII. Parallel to my main research I also worked in a collaboration to elucidate the total proteome of Nostoc sp. strain 7120 and Nostoc punctiforme using quantitative shotgun proteomics. Several hundred proteins were quantified for both species. It was possible to trace the detailed changes that occurred in the energy and nitrogen metabolism of a heterocyst after differentiation. Moreover, the presence of PSII proteins identified in our membrane proteome was also confirmed and extended. Lastly, I studied how the heterocysts are capable of responding to variations in light quality as compared to vegetative cells. Using 77 K fluorescence spectroscopy on heterocysts and vegetative cells previously illuminated with light at specific wavelengths, I was able to demonstrate that heterocysts still possess a possibly modified but functional antenna system, capable of harvesting light and transferring energy preferentially to PSI. The characterization of the membrane and total proteome permitted to draw a more comprehensive and integrated picture of the interplay between the distinct metabolic processes that are carried out in each cell type at the same time; from oxygenic photosynthesis and carbon fixation in the vegetative cells to the anoxygenic cyclic photophosphorylation essential to power nitrogen assimilation in the heterocysts.

sted, utgiver, år, opplag, sider
Uppsala: Acta Universitatis Upsaliensis, 2009. s. 78
Serie
Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Science and Technology, ISSN 1651-6214 ; 671
Emneord
Nostoc punctiforme, heterocyst, photosynthesis, thylakoid, isolation, proteomics, photosystem, energy transfer, hydrogen
HSV kategori
Forskningsprogram
biokemi
Identifikatorer
urn:nbn:se:uu:diva-108413 (URN)978-91-554-7607-6 (ISBN)
Disputas
2009-10-30, Häggsalen, Lägerhyddsvägen 1, Uppsala, 10:15 (engelsk)
Opponent
Veileder
Tilgjengelig fra: 2009-10-09 Laget: 2009-09-17 Sist oppdatert: 2009-10-09

Open Access i DiVA

Fulltekst mangler i DiVA

Andre lenker

Forlagets fulltekst

Person

Cardona, TanaiLindblad, PeterStensjö, Karin

Søk i DiVA

Av forfatter/redaktør
Cardona, TanaiLindblad, PeterStensjö, Karin
Av organisasjonen
I samme tidsskrift
Journal of Proteome Research

Søk utenfor DiVA

GoogleGoogle Scholar

doi
urn-nbn

Altmetric

doi
urn-nbn
Totalt: 1244 treff
RefereraExporteraLink to record
Permanent link

Direct link
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association
  • vancouver
  • Annet format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annet språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf