uu.seUppsala universitets publikasjoner
Endre søk
RefereraExporteraLink to record
Permanent link

Direct link
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association
  • vancouver
  • Annet format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annet språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf
Noise and colocalization in fluorescence microscopy: solving a problem
2008 (engelsk)Inngår i: Microscopy and Analysis, Vol. 22, nr 5, s. 7-10Artikkel, forskningsoversikt (Annet vitenskapelig) Published
Abstract [en]

The Pearson correlation coefficient (PCC) is regularly used in colocalization measurements, but it is sensitive to image noise. Images of fluorophores are usually degraded by Poisson and background noise and we have found that, even with apparently high quality images, the measured PCC is substantially understated, to the extent that the numbers become misleading. This means that ostensibly significant differences in the PCC between two populations may just reflect differing image quality while dissimilar levels of noise may mask significant differences. A new correction, based on measurements of image quality, derived from a pair of images for each fluorophore, aligns the measured PCC with the true PCC. Our method, the Replicate Based Noise Corrected Correlation (RBNCC), generates an accurate PCC even from poor images. It is highly photon efficient and therefore well suited for use in live cell fluorescence imaging or with rapidly bleaching fluorophores.

sted, utgiver, år, opplag, sider
2008. Vol. 22, nr 5, s. 7-10
HSV kategori
Identifikatorer
URN: urn:nbn:se:uu:diva-193109OAI: oai:DiVA.org:uu-193109DiVA, id: diva2:601343
Tilgjengelig fra: 2013-01-29 Laget: 2013-01-29 Sist oppdatert: 2013-07-12bibliografisk kontrollert

Open Access i DiVA

Fulltekst mangler i DiVA

Personposter BETA

Parmryd, Ingela

Søk i DiVA

Av forfatter/redaktør
Parmryd, Ingela

Søk utenfor DiVA

GoogleGoogle Scholar

urn-nbn

Altmetric

urn-nbn
Totalt: 693 treff
RefereraExporteraLink to record
Permanent link

Direct link
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association
  • vancouver
  • Annet format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annet språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf