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Auxotrophy and intrapopulation complementary in the "interactome' of a cultivated freshwater model community
Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för ekologi och genetik, Limnologi. Uppsala universitet, Science for Life Laboratory, SciLifeLab.
Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för ekologi och genetik, Limnologi. Uppsala universitet, Science for Life Laboratory, SciLifeLab.
Univ Wisconsin, Dept Bacteriol, Madison, WI 53706 USA.;Univ Wisconsin, Dept Civil & Environm Engn, Madison, WI 53706 USA..
Leibniz Inst Freshwater Ecol & Inland Fisheries, Dept Expt Limnol, D-16775 Ot Neuglobsow, Stechlin, Germany.;Univ Potsdam, Inst Biochem & Biol, D-14476 Potsdam, Germany..
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2015 (engelsk)Inngår i: Molecular Ecology, ISSN 0962-1083, E-ISSN 1365-294X, Vol. 24, nr 17, s. 4449-4459Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

Microorganisms are usually studied either in highly complex natural communities or in isolation as monoclonal model populations that we manage to grow in the laboratory. Here, we uncover the biology of some of the most common and yet-uncultured bacteria in freshwater environments using a mixed culture from Lake Grosse Fuchskuhle. From a single shotgun metagenome of a freshwater mixed culture of low complexity, we recovered four high-quality metagenome-assembled genomes (MAGs) for metabolic reconstruction. This analysis revealed the metabolic interconnectedness and niche partitioning of these naturally dominant bacteria. In particular, vitamin- and amino acid biosynthetic pathways were distributed unequally with a member of Crenarchaeota most likely being the sole producer of vitamin B12 in the mixed culture. Using coverage-based partitioning of the genes recovered from a single MAG intrapopulation metabolic complementarity was revealed pointing to social' interactions for the common good of populations dominating freshwater plankton. As such, our MAGs highlight the power of mixed cultures to extract naturally occurring interactomes' and to overcome our inability to isolate and grow the microbes dominating in nature.

sted, utgiver, år, opplag, sider
2015. Vol. 24, nr 17, s. 4449-4459
Emneord [en]
community, cultures, interactions, metagenomics, populations
HSV kategori
Identifikatorer
URN: urn:nbn:se:uu:diva-262971DOI: 10.1111/mec.13319ISI: 000360445700009PubMedID: 26179741OAI: oai:DiVA.org:uu-262971DiVA, id: diva2:856310
Forskningsfinansiär
Swedish Research Council, 2012-4592Tilgjengelig fra: 2015-09-23 Laget: 2015-09-23 Sist oppdatert: 2017-12-01bibliografisk kontrollert

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Garcia, Sarahi L.Buck, MoritzEiler, Alexander

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