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Investigation of interference from canine anti-mouse antibodies in hormone immunoassays
Department of Clinical Sciences, Swedish University of Agricultural Sciences, Uppsala, Sweden.
Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för medicinska vetenskaper, Klinisk kemi.ORCID-id: 0000-0003-3161-0402
Department of Clinical Sciences, Swedish University of Agricultural Sciences, Uppsala, Sweden.
Department of Clinical Sciences, Swedish University of Agricultural Sciences, Uppsala, Sweden.
2019 (Engelska)Ingår i: Veterinary clinical pathology, ISSN 0275-6382, E-ISSN 1939-165X, Vol. 48, nr S1, s. 59-69Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

BACKGROUND: Canine anti-mouse antibodies are a potential source of immunoassay interference, but erroneous immunoassay results are not always easily identifiable. Anti-Müllerian hormone (AMH) is a marker for the presence of gonads in dogs, but elevated AMH concentrations in neutered dogs could also be caused by antibody interference. For other assays, a discrepant result obtained after antibody precipitation might indicate antibody interference.

OBJECTIVES: We aimed to evaluate if canine anti-mouse antibodies are a source of erroneous results in the AMH assay and if antibody precipitation with polyethylene glycol (PEG) is a useful tool for detecting antibody interference in a variety of immunoassays used in the veterinary clinical laboratory.

METHODS: Twenty-nine positive and 25 negative samples for anti-mouse antibodies were analyzed for AMH, canine total thyroxine (TT4), canine thyroid-stimulating hormone (TSH) and progesterone before and after treatment with PEG. Results that differed by more than four SDs from the intra-assay coefficients of variation were considered discrepant. Elevated AMH concentrations in neutered dogs with anti-mouse antibodies and no visible gonads present were considered evidence of interference.

RESULTS: Evidence of antibody interference was found in two samples analyzed for AMH. The presence of anti-mouse antibodies did not lead to a higher proportion of discrepant results after PEG treatment for any of the immunoassays. The overall incidence of discrepant results for healthy controls was very high (73%).

CONCLUSIONS: Canine anti-mouse antibodies are a source of erroneous AMH results. Antibody precipitation with PEG is not a useful tool for detecting interference caused by such antibodies.

Ort, förlag, år, upplaga, sidor
2019. Vol. 48, nr S1, s. 59-69
Nyckelord [en]
PEG, anti-Müllerian hormone, antibody, canine, polyethylene glycol
Nationell ämneskategori
Klinisk vetenskap
Identifikatorer
URN: urn:nbn:se:uu:diva-389639DOI: 10.1111/vcp.12764ISI: 000476103200001PubMedID: 31318069OAI: oai:DiVA.org:uu-389639DiVA, id: diva2:1338041
Tillgänglig från: 2019-07-19 Skapad: 2019-07-19 Senast uppdaterad: 2019-12-06Bibliografiskt granskad

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