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Quantification of total and visceral adipose tissue in fructose-fed rats using water-fat separated single echo MRI
Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för medicinska vetenskaper, Arbets- och miljömedicin.
Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för medicinska vetenskaper, Arbets- och miljömedicin.ORCID-id: 0000-0002-8949-3555
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2013 (Engelska)Ingår i: Obesity, ISSN 1930-7381, E-ISSN 1930-739X, Vol. 21, nr 9, s. E388-E395Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Objective: The aim of this study was to setup a rodent model for modest weight gain and an MRI-based quantification of body composition on a clinical 1.5 T MRI system for studies of obesity and environmental factors and their possible association. Design and Methods: Twenty-four 4-week-old female Fischer rats were divided into two groups: one exposed group (n=12) and one control group (n 12). The exposed group was given drinking water containing fructose (5% for 7 weeks, then 20% for 3 weeks). The control group was given tap water. Before sacrifice, whole body MRI was performed to determine volumes of total and visceral adipose tissue and lean tissue. MRI was performed using a clinical 1.5 T system and a chemical shift based technique for separation of water and fat signal from a rapid single echo acquisition. Fat signal fraction was used to separate adipose and lean tissue. Visceral adipose tissue volume was quantified using semiautomated segmentation. After sacrifice, a perirenal fat pad and the liver were dissected and weighed. Plasma proteins were analyzed by Western blot. Results: The weight gain was 5.2% greater in rats exposed to fructose than in controls (P=0.042). Total and visceral adipose tissue volumes were 5.2 cm(3) (P=0.017) and 3.1 cm(3) (P=0.019) greater, respectively, while lean tissue volumes did not differ. The level of triglycerides and apolipoprotein A-I was higher (P=0.034, P=0.005, respectively) in fructose-exposed rats.

Ort, förlag, år, upplaga, sidor
2013. Vol. 21, nr 9, s. E388-E395
Nationell ämneskategori
Medicinsk bildbehandling Medicin och hälsovetenskap
Identifikatorer
URN: urn:nbn:se:uu:diva-209508DOI: 10.1002/oby.20229ISI: 000325426600007OAI: oai:DiVA.org:uu-209508DiVA, id: diva2:658149
Tillgänglig från: 2013-10-21 Skapad: 2013-10-21 Senast uppdaterad: 2017-12-06Bibliografiskt granskad
Ingår i avhandling
1. Environmental Contaminants and Obesity
Öppna denna publikation i ny flik eller fönster >>Environmental Contaminants and Obesity
2013 (Engelska)Doktorsavhandling, sammanläggning (Övrigt vetenskapligt)
Abstract [en]

Obesity is a worldwide problem affecting both children and adults. Genetic, physiological, environmental, psychological, social and economic factors interact in varying degrees, influencing body weight and fat distribution and the progress of obesity. Moreover, some anthropogenic chemicals have proven to be endocrine disrupting chemicals (EDCs) with the potential to interfere with different actions of hormones in the body. EDCs may thereby disrupt homeostasis, modifying developmental, behavioral and immune functions in humans and animals, and also promoting adiposity. Because hormones generally act at low concentrations, small changes in the endocrine system may lead to extensive effects. Based on data from experimental and epidemiological studies this thesis elucidates the relationship between a large number of environmental contaminants and obesity.

The experimental studies demonstrated that fructose supplementation in the drinking water resulted in unfavorable metabolic alterations such as a higher liver somatic index (LSI), an increase in plasma triglycerides and increased plasma levels of apo A-I. Fructose in combination with exposure to bisphenol A (BPA) increased liver fat content and plasma levels of apo A-I in juvenile female Fischer 344 rats. The experimental studies also showed that the retro-peritoneal fat, which in rats is a distinct fat depot easy to distinguish and dissect, correlated well with the measurements of total fat mass analyzed with MRI, and could therefore be used as a substitute for total fat mass in rats.

The epidemiological studies showed that circulating levels of persistent organic pollutants (POPs) were related to fat mass measured by DXA. OCDD, HCB, TNC, DDE and the less chlorinated PCBs were positively related to fat mass, while the more highly chlorinated PCBs showed a negative association. Further, circulating levels of BPA were positively associated with levels of the hormones adiponectin and leptin, but negatively related with ghrelin, hormones which are involved in the regulation of hunger and satiety. However, serum BPA levels were not related to measures of fat mass in the elderly individuals in the PIVUS cohort.

This thesis concludes that environmental contaminants such as BPA and POPs most likely are contributors, along with genetic, social and behavioral factors, to the development of obesity.

Ort, förlag, år, upplaga, sidor
Uppsala: Acta Universitatis Upsaliensis, 2013. s. 67
Serie
Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Medicine, ISSN 1651-6206 ; 949
Nyckelord
Fischer 344, rat, obesity, adipose tissue, persistent organic pollutants, POPs, bisphenol A, BPA, pesticides, dioxin, PCB, DDT, apo A-I, adiponectin, leptin, ghrelin
Nationell ämneskategori
Medicin och hälsovetenskap
Identifikatorer
urn:nbn:se:uu:diva-209807 (URN)978-91-554-8798-0 (ISBN)
Disputation
2013-12-13, Frödingsalen, Ulleråkersvägen 40 A, Uppsala, 13:15 (Svenska)
Opponent
Handledare
Tillgänglig från: 2013-11-20 Skapad: 2013-10-26 Senast uppdaterad: 2016-11-04

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Rönn, MonikaLind, Monica P.Berglund, JohanMalmberg, FilipÖrberg, JanLind, LarsOrtiz-Nieto, FranciscoKullberg, Joel

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Rönn, MonikaLind, Monica P.Berglund, JohanMalmberg, FilipÖrberg, JanLind, LarsOrtiz-Nieto, FranciscoKullberg, Joel
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Arbets- och miljömedicinEnheten för radiologiBildanalys och människa-datorinteraktionAvdelningen för visuell information och interaktionMiljötoxikologiKardiovaskulär epidemiologi
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