Hydrogel Patterning by Diffusion through the Matrix and Subsequent Light-Triggered Chemical ImmobilizationVisa övriga samt affilieringar
2015 (Engelska)Ingår i: ACS Applied Materials and Interfaces, ISSN 1944-8244, E-ISSN 1944-8252, Vol. 7, nr 2, s. 1194-1206Artikel i tidskrift (Refereegranskat) Published
Abstract [en]
A novel approach to hyaluronic acid (HA) hydrogel with a chemical gradient of the matrix-linked bisphosphonate (BP) groups is presented. The method consists of two steps, including initial generation of physical gradient patterns of BPs by diffusion of BP acrylamide reagent into HA matrix carrying thiol groups and subsequent chemical immobilization of the BP groups by UV light-triggered thiolene addition reaction. This gradient hydrogel permits spatial three-dimensional regulation of secondary interactions of different molecules with the polymer matrix. In particular, graded amounts of cytochrome c (cyt c) were reversibly absorbed in the hydrogel, thus enabling the subsequent spatially controlled release of the therapeutic protein. The obtained patterned hydrogel acts also as a unique reactor in which peroxidase-catalyzed oxidation of a substrate is determined by spatial position of the enzyme (cyt c) in the matrix resulting in a range of product concentrations. As an example, matrix template-assisted oxidation of 3,3',5,5'-tetarmethylbenzydine (TMB) in the presence of H2O2 occurs simultaneously at different rates within the gradient hydrogel. Moreover, calcium binding to the gradient HABP hydrogel reflects the pattern of immobilized BP groups eventually leading to the graded biomineralization of the matrix. This approach opens new possibilities for use of hydrogels as dynamic models for biologic three-dimensional structures such as extracellular matrix.
Ort, förlag, år, upplaga, sidor
2015. Vol. 7, nr 2, s. 1194-1206
Nyckelord [en]
hydrogel, chemical gradient, template synthesis, orthogonal reactions, hyaluronic acid
Nationell ämneskategori
Kemi
Identifikatorer
URN: urn:nbn:se:uu:diva-247160DOI: 10.1021/am506926wISI: 000348332700020PubMedID: 25575380OAI: oai:DiVA.org:uu-247160DiVA, id: diva2:795529
2015-03-162015-03-132022-01-28Bibliografiskt granskad