uu.seUppsala universitets publikationer
Ändra sökning
RefereraExporteraLänk till posten
Permanent länk

Direktlänk
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association
  • vancouver
  • Annat format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annat språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf
Protein Desalination Chip for Mass Spectrometry Sample Preparation
Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Tekniska sektionen, Institutionen för teknikvetenskaper, Mikrosystemteknik.
Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Kemiska sektionen, Institutionen för kemi - BMC, Analytisk kemi.
Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Tekniska sektionen, Institutionen för teknikvetenskaper, Mikrosystemteknik.
(3Dept. Mechanical Engineering, Sogang University, 35 Baekbeom-ro, Mapo-gu, Seoul 121-742, KOREA)
Visa övriga samt affilieringar
2015 (Engelska)Konferensbidrag, Muntlig presentation med publicerat abstract (Refereegranskat)
Abstract [en]

This work focuses on desalination of a protein sample in a lab-on-chip device using the ion concentration polarization (ICP) technique. It was demonstrated with a salt containing buffer with four proteins and two peptides of concentrations typical to cerebrospinal fluid (CSF). Not only was the output desalinated but its protein concentration with large molecular weight (MW) was as much as 3 times higher for the largest protein compared to the original. We conclude that ICP based microfluidic chips have great potential for desalination and protein concentration in microdialysis sampling coupled to mass spectroscopy (MS).

Ort, förlag, år, upplaga, sidor
MicroTAS, 2015.
Nyckelord [en]
Ion concentration polarization, Desalination, Protein, Mass spectroscopy
Nationell ämneskategori
Nanoteknik
Forskningsämne
Teknisk fysik med inriktning mot mikrosystemteknik; Teknisk fysik med inriktning mot materialvetenskap
Identifikatorer
URN: urn:nbn:se:uu:diva-261067OAI: oai:DiVA.org:uu-261067DiVA: diva2:849551
Konferens
The 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015. October 25-29, 2015. Gyeongju, Korea.
Tillgänglig från: 2015-08-28 Skapad: 2015-08-28 Senast uppdaterad: 2016-01-05
Ingår i avhandling
1. Microdialysis Sampling of Macro Molecules: Fluid Characteristics, Extraction Efficiency and Enhanced Performance
Öppna denna publikation i ny flik eller fönster >>Microdialysis Sampling of Macro Molecules: Fluid Characteristics, Extraction Efficiency and Enhanced Performance
2015 (Engelska)Doktorsavhandling, sammanläggning (Övrigt vetenskapligt)
Abstract [en]

In this thesis, fluid characteristics and sampling efficiency of high molecular weight cut-off microdialysis are presented, with the aim of improving the understanding of microdialysis sampling mechanisms and its performance regarding extraction efficiency of biological fluid and biomarkers.

Microdialysis is a well-established clinical sampling tool for monitoring small biomarkers such as lactate and glucose. In recent years, interest has raised in using high molecular weight cut-off microdialysis to sample macro molecules such as neuropeptides, cytokines and proteins. However, with the increase of the membrane pore size, high molecular weight cut-off microdialysis exhibits drawbacks such like unstable catheter performance, imbalanced fluid recovery, low and unstable molecule extraction efficiency, etc. But still, the fluid characteristics of high molecular weight cut-off microdialysis is rarely studied, and the clinical or in vitro molecule sampling efficiency from recent studies vary from each other and are difficult to compare.  

Therefore, in this thesis three aspects of high molecular weight cut-off microdialysis have been explored. The first, the fluid characteristics of large pore microdialysis has been investigated, theoretically and experimentally. The results suggest that the experimental fluid recovery is in consistency with its theoretical formula. The second, the macromolecule transport behaviour has been visualized and semi-quantified, using an in vitro test system and fluorescence imaging. The third, two in vitro tests have been done to mimic in vivo cerebrospinal fluid sampling under pressurization, using native and differently surface modified catheters. As results, individual protein/peptide extraction efficiencies were achieved, using targeted mass spectrometry analysis.

In summary, a theory system of the fluid characteristics of high molecular weight cut-off microdialysis has been built and testified; Macromolecular transport of microdialysis catheter has been visualized; In vivo biomolecules sampling has been simulated by well-defined in vitro studies; Individual biomolecular extraction efficiency has been shown; Different surface modifications of microdialysis catheter have been investigated. It was found that, improved sampling performance can be achieved, in terms of balanced fluid recovery and controlled protein extraction efficiency.

Ort, förlag, år, upplaga, sidor
Uppsala: Acta Universitatis Upsaliensis, 2015. 52 s.
Serie
Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Science and Technology, ISSN 1651-6214 ; 1278
Nyckelord
microdialysis, high molecular weight cut-off, fluid characteristics, fluid recovery, extraction efficiency, biomarker, microporous membrane, macromolecule transport, transmembrane, large pore, surface modification, pluronic, dextran, in vitro, microdialysis catheter
Nationell ämneskategori
Bearbetnings-, yt- och fogningsteknik Nanoteknik
Forskningsämne
Teknisk fysik med inriktning mot mikrosystemteknik; Teknisk fysik med inriktning mot materialvetenskap
Identifikatorer
urn:nbn:se:uu:diva-261068 (URN)978-91-554-9315-8 (ISBN)
Disputation
2015-10-16, Polhem Salen, Angstrom Laboratory, Uppsala, 09:15 (Engelska)
Opponent
Handledare
Forskningsfinansiär
Berzelii Centre EXSELENT
Tillgänglig från: 2015-09-25 Skapad: 2015-08-28 Senast uppdaterad: 2015-10-01

Open Access i DiVA

Fulltext saknas

Sök vidare i DiVA

Av författaren/redaktören
Chu, JiangtaoUndin, TorgnyDahlin, AndreasHjort, Klas
Av organisationen
MikrosystemteknikAnalytisk kemi
Nanoteknik

Sök vidare utanför DiVA

GoogleGoogle Scholar

Totalt: 1411 träffar
RefereraExporteraLänk till posten
Permanent länk

Direktlänk
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association
  • vancouver
  • Annat format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annat språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf