HAdV-2-suppressed growth of SV40 T antigen-transformed mouse mammary epithelial cell-induced tumours in SCID miceVisa övriga samt affilieringar
2016 (Engelska)Ingår i: Virology, ISSN 0042-6822, E-ISSN 1096-0341, Vol. 489, s. 44-50Artikel i tidskrift (Refereegranskat) Published
Resurstyp
Text
Abstract [en]
Human adenovirus (HAdV) vectors are promising tools for cancer therapy, but the shortage of efficient animal models for productive HAdV infections has restricted the evaluation of systemic effects to mainly immunodeficient mice. Previously, we reported a highly efficient replication of HAdV-2 in a non-tumorigenic mouse mammary epithelial cell line, NMuMG. Here we show that HAdV-2 gene expression and progeny formation in NMuMG cells transformed with the SV40 T antigen (NMuMG-T cells) were as efficient as in the parental NMuMG cells. Injection of HAdV-2 into tumours established by NMuMG-T in SCID mice caused reduced tumour growth and signs of intratumoural lesions. HAdV-2 replicated within the NMuMG-T-established tumours, but not in interspersed host-derived tissues within the tumours. The specific infection of NMuMG-T-derived tumours was verified by the lack of viral DNA in kidney, lung or spleen although low levels of viral DNA was occasionally found in liver.
Ort, förlag, år, upplaga, sidor
2016. Vol. 489, s. 44-50
Nyckelord [en]
Oncolytic adenovirus, Murine cells, Anti-tumour activity, Permissive infection, Viral replication
Nationell ämneskategori
Infektionsmedicin
Identifikatorer
URN: urn:nbn:se:uu:diva-282389DOI: 10.1016/j.virol.2015.11.031ISI: 000370892100005PubMedID: 26707269OAI: oai:DiVA.org:uu-282389DiVA, id: diva2:917040
Forskningsfinansiär
Cancerfonden2016-04-052016-04-052022-01-29Bibliografiskt granskad