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The voltage-gated proton channel Hv1/VSOP inhibits neutrophil granule release
Osaka Univ, Grad Sch Med, Integrat Physiol, 2-2 Yamada Oka, Suita, Osaka 5650871, Japan..
Yokohama City Univ, Dept Life & Environm Syst Sci, Grad Sch Nanobiosci, Yokohama, Kanagawa, Japan..
Hosp Sick Children, Physiol & Expt Med, 555 Univ Ave, Toronto, ON M5G 1X8, Canada..
Osaka Univ, Grad Sch Med, Integrat Physiol, 2-2 Yamada Oka, Suita, Osaka 5650871, Japan..
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2016 (Engelska)Ingår i: Journal of Leukocyte Biology, ISSN 0741-5400, E-ISSN 1938-3673, Vol. 99, nr 1, s. 7-19Artikel i tidskrift (Refereegranskat) Published
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Abstract [en]

Neutrophil granule exocytosis is crucial for host defense and inflammation. Neutrophils contain 4 types of granules, the exocytotic release of which is differentially regulated. This exocytosis is known to be driven by diverse mediators, including calcium and nucleotides, but the precise molecular mechanism remains largely unknown. We show in the present study that voltage-gated proton (Hv) channels are necessary for the proper release of azurophilic granules in neutrophils. On activation of NADPH oxidase by PMA and IgG, neutrophils derived from Hvcn1 gene knockout mouse exhibited greater secretion of MPO and elastase than WT cells. In contrast, release of LTF enriched in specific granules was not enhanced in these cells. The excess release of azurophilic granules in Hv1/VSOP-deficient neutrophils was suppressed by inhibiting NADPH oxidase activity and, in part, by valinomycin, a potassium ionophore. In addition, Hv1/VSOP-deficient mice exhibited more severe lung inflammation after intranasal Candida albicans infection than WT mice. These findings suggest that the Hv channel acts to specifically dampen the release of azurophilic granules through, in part, the suppression of increased positive charges at the plasma membrane accompanied by the activation of NADPH oxidase in neutrophils.

Ort, förlag, år, upplaga, sidor
2016. Vol. 99, nr 1, s. 7-19
Nyckelord [en]
proton channel, inflammation
Nationell ämneskategori
Medicin och hälsovetenskap
Identifikatorer
URN: urn:nbn:se:uu:diva-283809DOI: 10.1189/jlb.3HI0814-393RISI: 000371890000003PubMedID: 25990245OAI: oai:DiVA.org:uu-283809DiVA, id: diva2:919677
Tillgänglig från: 2016-04-14 Skapad: 2016-04-14 Senast uppdaterad: 2017-11-30Bibliografiskt granskad

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