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Stanniocalcin-1 expression in normal human endometrium and dysregulation in endometriosis
Univ Calif San Francisco, Dept Obstet Gynecol & Reprod Sci, 550 16th St,7th Floor,Box 0132, San Francisco, CA 94158 USA..
Competence Ctr Hlth Technol, Tartu, Estonia.;Univ Granada, Sch Med, Dept Pediat, Granada, Spain..
Competence Ctr Hlth Technol, Tartu, Estonia.;Univ Tartu, Inst Technol, Prote Core Facil, Tartu, Estonia.;Univ Tartu, Womens Clin, Tartu, Estonia..
Competence Ctr Hlth Technol, Tartu, Estonia.;Univ Tartu, Womens Clin, Tartu, Estonia..
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2016 (Engelska)Ingår i: Fertility and Sterility, ISSN 0015-0282, E-ISSN 1556-5653, Vol. 106, nr 3, s. 681-691Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Objective: To determine expression of stanniocalcin-1 (STC1) in human endometrium with and without endometriosis and its regulation by steroid hormones. Design: Laboratory study. Setting: University. Patient(s): Nineteen women with endometriosis and 33 control women. Intervention(s): Endometrial biopsy and fluid sampling. Main Outcome Measure(s): Analysis of early secretory (ESE) and midsecretory (MSE) endometrial secretomes from fertile women with the use of nano-liquid chromatography-dual mass spectrometry;real-time quantitative polymerase chain reaction, and immunohistochemistry for STC1 and its receptor calcium-sensing receptor (CASR) mRNA and proteins in endometrium with and without endometriosis; evaluation of STC1 and CASR mRNA expression in endometrial stromal fibroblasts (eSF) from women with and without endometriosis decidualized with the use of E2P or 8-bromo-cyclic adenosine monophosphate (cAMP). Result(s): STC1 protein was strongly up-regulated in MSE versus ESE in endometrial fluid of fertile women. STC1 mRNA significantly increased in MSE from women with, but not from those without, endometriosis, compared with proliferative endometrium or ESE, with no significant difference throughout the menstrual cycle between groups. STC1 mRNA in eSF from control women increased >230-fold on decidualization with the use of cAMP versus 45-fold from women with endometriosis, which was not seen on decidualization with E-2/P. CASR mRNA did not exhibit significant differences in any condition and was not expressed in isolated eSF. STC1 protein immunoexpression in eSF was significantly lower in women with endometriosis compared with control women. Conclusion(s): STC1 protein is significantly up-regulated in MSE endometrial fluid and is dysregulated in eutopic endometrial tissue from women with endometriosis. It is likely regulated by cAMP and may be involved in the pathogenesis of decidualization defects.

Ort, förlag, år, upplaga, sidor
2016. Vol. 106, nr 3, s. 681-691
Nyckelord [en]
Stanniocalcin-1, human endometrium, endometriosis, stromal fibroblasts, decidualization, proteomics
Nationell ämneskategori
Medicin och hälsovetenskap
Identifikatorer
URN: urn:nbn:se:uu:diva-308947DOI: 10.1016/j.fertnstert.2016.05.023ISI: 000386569800034PubMedID: 27322879OAI: oai:DiVA.org:uu-308947DiVA, id: diva2:1051390
Forskningsfinansiär
EU, FP7, Sjunde ramprogrammet, EU41564, EU324509Svenska läkaresällskapetTillgänglig från: 2016-12-01 Skapad: 2016-12-01 Senast uppdaterad: 2017-11-29Bibliografiskt granskad

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