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Sensitive magnetic biodetection using magnetic multi-core nanoparticles and RCA coils
Vise andre og tillknytning
2017 (engelsk)Inngår i: Journal of Magnetism and Magnetic Materials, ISSN 0304-8853, E-ISSN 1873-4766, Vol. 427, s. 14-18Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

We use functionalized iron oxide magnetic multi-core particles of 100 nm in size (hydrodynamic particle diameter) and AC susceptometry (ACS) methods to measure the binding reactions between the magnetic nanoparticles (MNPs) and bio-analyte products produced from DNA segments using the rolling circle amplification (RCA) method. We use sensitive induction detection techniques in order to measure the ACS response. The DNA is amplified via RCA to generate RCA coils with a specific size that is dependent on the amplification time. After about 75 min of amplification we obtain an average RCA coil diameter of about 1 µm. We determine a theoretical limit of detection (LOD) in the range of 11 attomole (corresponding to an analyte concentration of 55 fM for a sample volume of 200 µL) from the ACS dynamic response after the MNPs have bound to the RCA coils and the measured ACS readout noise. We also discuss further possible improvements of the LOD.

sted, utgiver, år, opplag, sider
Elsevier, 2017. Vol. 427, s. 14-18
Emneord [en]
Magnetic nanoparticles, Magnetic multi-core particles, AC susceptometry, RCA coil, Differential induction coil, Biodetection
HSV kategori
Forskningsprogram
Teknisk fysik med inriktning mot nanoteknologi och funktionella material
Identifikatorer
URN: urn:nbn:se:uu:diva-310469DOI: 10.1016/j.jmmm.2016.10.041ISI: 000397199400004OAI: oai:DiVA.org:uu-310469DiVA, id: diva2:1057036
Forskningsfinansiär
Knut and Alice Wallenberg FoundationSwedish Foundation for Strategic Research , SBE13-0125EU, FP7, Seventh Framework Programme, 604448Tilgjengelig fra: 2016-12-16 Laget: 2016-12-16 Sist oppdatert: 2019-02-27bibliografisk kontrollert

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Zardán Gómez de la Torre, TeresaStrømme, Maria

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