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Clinical implications of assay specific differences in f-calprotectin when monitoring inflammatory bowel disease activity over time
Univ Orebro, Fac Med & Hlth, Dept Gastroenterol, SE-70182 Orebro, Sweden..
Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för medicinska vetenskaper, Biokemisk endokrinologi.ORCID-id: 0000-0002-9198-4193
Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för medicinska vetenskaper, Gastroenterologi/hepatologi.
Univ Orebro, Sch Med Sci, Clin Epidemiol & Biostat, Orebro, Sweden..
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2017 (Engelska)Ingår i: Scandinavian Journal of Gastroenterology, ISSN 0036-5521, E-ISSN 1502-7708, Vol. 52, nr 3, s. 344-350Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Objective: With several faecal calprotectin (FC) assays on the market, it has been difficult to define a uniform threshold for discriminating between remission and active disease in patients with inflammatory bowel disease (IBD). We aimed to compare the results of different FC-assays in IBD patients, followed over time.Material and methods: IBD patients provided faecal samples and reported clinical activity every third month prospectively over a two year period. FC was measured with two ELISA - (Buhlmann and Immunodiagnostik) and one automated fluoroimmunoassay (Phadia).Results: In total, 13 patients provided 91 faecal samples. The median (IQR) concentration of FC was higher at active disease than at remission for all assays: Buhlmann 845 (1061-226) g/g versus 62 (224-39) g/g, Phadia 369 (975-122) g/g versus 11 (52-11) g/g, and Immundiagnostik 135 (302-69) g/g versus 8 (56-4) g/g. The Buhlmann assay produced the largest absolute difference but the corresponding relative difference seemed to be more pronounced when analysed by the Phadia - (ratio of means 8.5; 95% CI 3.3-21.9) or the Immundiagnostik assay (ratio of means 7.4; 95% CI 3.1-17.6) than by the Buhlmann assay (ratio of means 5.3; 95% CI 2.7-10.6). Consequently, the specificity for discriminating active disease from remission varied between assays (34-75%) when the cut-off 50g/g was used, whereas the differences in sensitivity were less pronounced.Conclusions: Cross-comparisons revealed overall poor agreement between the assays as well as differences in the dynamics of FC. These findings suggest that standardisation of the method is needed to implement FC as a disease monitoring tool at large-scale.

Ort, förlag, år, upplaga, sidor
2017. Vol. 52, nr 3, s. 344-350
Nyckelord [en]
Biomarker, Crohn's disease, faecal calprotectin, inflammatory bowel disease, ulcerative colitis
Nationell ämneskategori
Gastroenterologi
Identifikatorer
URN: urn:nbn:se:uu:diva-316426DOI: 10.1080/00365521.2016.1256424ISI: 000392488800015PubMedID: 27881032OAI: oai:DiVA.org:uu-316426DiVA, id: diva2:1077931
Forskningsfinansiär
Vetenskapsrådet, 521-2011-2764Tillgänglig från: 2017-03-01 Skapad: 2017-03-01 Senast uppdaterad: 2017-11-29Bibliografiskt granskad

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Stridsberg, MatsLampinen, MariaCarlson, Marie

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