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Nanometer resolution imaging and tracking of fluorescent molecules with minimal photon fluxes
Max Planck Inst Biophys Chem, Dept NanoBiophoton, Gottingen, Germany..
Max Planck Inst Biophys Chem, Dept NanoBiophoton, Gottingen, Germany..
Max Planck Inst Biophys Chem, Dept NanoBiophoton, Gottingen, Germany..
Uppsala universitet, Science for Life Laboratory, SciLifeLab. Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för cell- och molekylärbiologi, Molekylär systembiologi.
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2017 (engelsk)Inngår i: Science, ISSN 0036-8075, E-ISSN 1095-9203, Vol. 355, nr 6325, s. 606-612Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

We introduce MINFLUX, a concept for localizing photon emitters in space. By probing the emitter with a local intensity minimum of excitation light, MINFLUX minimizes the fluorescence photons needed for high localization precision. In our experiments, 22 times fewer fluorescence photons are required as compared to popular centroid localization. In superresolutionmicroscopy, MINFLUXattained similar to 1-nanometer precision, resolving molecules only 6 nanometers apart. MINFLUX tracking of single fluorescent proteins increased the temporal resolution and the number of localizations per trace by a factor of 100, as demonstrated with diffusing 30S ribosomal subunits in living Escherichia coli. As conceptual limits have not been reached, we expect this localization modality to break new ground for observing the dynamics, distribution, and structure of macromolecules in living cells and beyond.

sted, utgiver, år, opplag, sider
2017. Vol. 355, nr 6325, s. 606-612
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Identifikatorer
URN: urn:nbn:se:uu:diva-317586DOI: 10.1126/science.aak9913ISI: 000393636700043PubMedID: 28008086OAI: oai:DiVA.org:uu-317586DiVA, id: diva2:1083812
Forskningsfinansiär
EU, European Research CouncilKnut and Alice Wallenberg FoundationTilgjengelig fra: 2017-03-22 Laget: 2017-03-22 Sist oppdatert: 2017-11-29bibliografisk kontrollert

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