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Improved positioning and detectability of microparticles in droplet microfluidics using two-dimensional acoustophoresis
Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Tekniska sektionen, Institutionen för teknikvetenskaper, Mikrosystemteknik. (EMBLA)ORCID-id: 0000-0002-7023-4772
Lund University, Lund, Sweden. (EMBLA)ORCID-id: 0000-0001-7980-376X
Tech Univ Denmark, Lyngby, Denmark.ORCID-id: 0000-0001-5827-2939
Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Tekniska sektionen, Institutionen för teknikvetenskaper, Mikrosystemteknik. Uppsala universitet, Science for Life Laboratory, SciLifeLab. Lund University, Lund, Sweden. (EMBLA)ORCID-id: 0000-0002-1264-1337
2017 (engelsk)Inngår i: Journal of Micromechanics and Microengineering, ISSN 0960-1317, E-ISSN 1361-6439, Vol. 27, nr 8, artikkel-id 084002Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

We have fabricated a silicon-glass two-phase droplet microfluidic system capable of generating sub 100 µm-sized,   =  (74  ±  2) µm, spherical droplets at rates of up to hundreds of hertz. By implementing a two-dimensional (2D) acoustophoresis particle-positioning method, we show a fourfold improvement in both vertical and lateral particle positioning inside the droplets compared to unactuated operation. The efficiency of the system has been optimized by incorporating aluminum matching layers in the transducer design permitting biocompatible operational temperatures (<37 °C). Furthermore, by using acoustic actuation, (99.8  ±  0.4)% of all encapsulated microparticles can be detected compared to only (79.0  ±  5.1)% for unactuated operation. In our experiments we observed a strong ordering of the microparticles in distinct patterns within the droplet when using 2D acoustophoresis; to explain the origin of these patterns we simulated numerically the fluid flow inside the droplets and compared with the experimental findings.

sted, utgiver, år, opplag, sider
2017. Vol. 27, nr 8, artikkel-id 084002
HSV kategori
Forskningsprogram
Teknisk fysik med inriktning mot mikrosystemteknik
Identifikatorer
URN: urn:nbn:se:uu:diva-327204DOI: 10.1088/1361-6439/aa7967ISI: 000415698300001OAI: oai:DiVA.org:uu-327204DiVA, id: diva2:1129793
Forskningsfinansiär
The Crafoord Foundation, 20130569Carl Tryggers foundation , CTS14478Swedish Research Council, 612-2013-5920Tilgjengelig fra: 2017-08-07 Laget: 2017-08-07 Sist oppdatert: 2018-02-26bibliografisk kontrollert

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