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Dissection and Culture of Mouse Embryonic Kidney
Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för neurovetenskap, Genetisk utvecklingsbiologi.ORCID-id: 0000-0002-1386-1307
Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för organismbiologi, Evolution och utvecklingsbiologi.
2017 (Engelska)Ingår i: Journal of Visualized Experiments, ISSN 1940-087X, E-ISSN 1940-087X, nr 123, artikel-id e55715Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

The goal of this protocol is to describe a method for the dissection, isolation, and culture of mouse metanephric rudiments. During mammalian kidney development, the two progenitor tissues, the ureteric bud and the metanephric mesenchyme, communicate and reciprocally induce cellular mechanisms to eventually form the collecting system and the nephrons of the kidney. As mammalian embryos grow intrauterine and therefore are inaccessible to the observer, an organ culture has been developed. With this method, it is possible to study epithelial-mesenchymal interactions and cellular behavior during kidney organogenesis. Furthermore, the origin of congenital kidney and urogenital tract malformations can be investigated. After careful dissection, the metanephric rudiments are transferred onto a filter that floats on culture medium and can be kept in a cell culture incubator for several days. However, one must be aware that the conditions are artificial and could influence the metabolism in the tissue. Also, the penetration of test substances could be limited due to the extracellular matrix and basal membrane present in the explant. One main advantage of organ culture is that the experimenter can gain direct access to the organ. This technology is cheap, simple, and allows a large number of modifications, such as the addition of biologically active substances, the study of genetic variants, and the application of advanced imaging techniques.

Ort, förlag, år, upplaga, sidor
2017. nr 123, artikel-id e55715
Nyckelord [en]
Developmental Biology, Issue 123, organogenesis, organ culture, kidney, metanephros, mouse
Nationell ämneskategori
Utvecklingsbiologi
Identifikatorer
URN: urn:nbn:se:uu:diva-331946DOI: 10.3791/55715ISI: 000406213900074OAI: oai:DiVA.org:uu-331946DiVA, id: diva2:1151568
Tillgänglig från: 2017-10-23 Skapad: 2017-10-23 Senast uppdaterad: 2017-11-29Bibliografiskt granskad

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