This article reports on a video-based analysis of bicycling practices in Gothenburg and Toulouse. It is based on actor-network theory, an approach that studies human and non-human entities and their contributions to social action equally. The paper examines bicycles and their interactions with cyclists and loads in the transport of people and goods. Accordingly, this paper presents methodological, theoretical and empirical contributions to the study of bicycle transportation as a possible method for developing sustainable urban environments. This paper also presents an innovative way to study ordinary social practices and describes how these practices shape associated societal issues.