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Comparisons between commercial salivary testosterone enzyme-linked immunosorbent assay kits
Orebro Univ, Dept Neurol, Fac Med & Hlth, Orebro, Sweden..
Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Kemiska sektionen, Institutionen för kemi - BMC, Analytisk kemi.ORCID-id: 0000-0002-4597-041x
Linkoping Univ, Dept Clin Chem, Linkoping, Sweden.;Linkoping Univ, Dept Clin & Expt Med, Linkoping, Sweden..
Orebro Univ, Dept Neurol, Fac Med & Hlth, Orebro, Sweden.;Linkoping Univ, Dept Clin Chem, Linkoping, Sweden.;Linkoping Univ, Dept Clin & Expt Med, Linkoping, Sweden..
2017 (Engelska)Ingår i: Scandinavian Journal of Clinical and Laboratory Investigation, ISSN 0036-5513, E-ISSN 1502-7686, Vol. 77, nr 8, s. 582-586Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Introduction: Measuring testosterone concentrations is of interest both in clinical situations and for research, the latter expanding rapidly during recent years. An increased demand for convenient methods has prompted a number of companies to develop enzyme-linked immunosorbent assay (ELISA) kits to measure testosterone concentrations in saliva. However, the inter-comparability of kits from different manufacturers have yet to be determined. Aim of study: The aim of this study was to compare commercially available ELISA kits from four different manufacturers (Salimetrics, IBL, DRG and Demeditec). Methods: Saliva was collected from 50 participants (25 men and 25 women). Each sample was analysed by the four ELISA kits. Results: The correlations between the ELISA kits from Demeditec, DRG and Salimetrics were moderate to high with r-values >.77; however, proportional errors between the methods calls for caution. The ELISA kit from IBL malfunctioned and no results from this kit was obtained. Conclusions: Results from studies using the ELISA kits from Demeditec, DRG and Salimetrics are generally comparable; however, translation using the formulae presented in the current study could increase the accuracy of these comparisons.

Ort, förlag, år, upplaga, sidor
2017. Vol. 77, nr 8, s. 582-586
Nyckelord [en]
Androgens, immunoassay, methods, saliva, testosterone
Nationell ämneskategori
Analytisk kemi
Identifikatorer
URN: urn:nbn:se:uu:diva-343341DOI: 10.1080/00365513.2017.1339231ISI: 000416756100004PubMedID: 28644096OAI: oai:DiVA.org:uu-343341DiVA, id: diva2:1189565
Forskningsfinansiär
Vetenskapsrådet, 2015-4870Tillgänglig från: 2018-03-12 Skapad: 2018-03-12 Senast uppdaterad: 2018-03-15Bibliografiskt granskad

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