uu.seUppsala University Publications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
GABA Regulates Release of Inflammatory Cytokines From Peripheral Blood Mononuclear Cells and CD4+ T Cells and Is Immunosuppressive in Type 1 Diabetes
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Neuroscience, Physiology.ORCID iD: 0000-0002-7116-0939
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Neuroscience, Physiology. (Molecular Physiology and Neuroscience)ORCID iD: 0000-0002-4717-1558
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Neuroscience, Physiology. Uppsala University. (Molecular Physiology and Neuroscience)ORCID iD: 0000-0001-8279-2790
Uppsala University, Science for Life Laboratory, SciLifeLab. Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Immunology, Genetics and Pathology, Molecular tools.
Show others and affiliations
2018 (English)In: EBioMedicine, ISSN 0360-0637, E-ISSN 2352-3964, Vol. 30, p. 283-294Article in journal (Refereed) Published
Abstract [en]

The neurotransmitter γ-aminobutyric acid (GABA) is an extracellular signaling molecule in the brain and in pancreatic islets. Here, we demonstrate that GABA regulates cytokine secretion from human peripheral blood mononuclear cells (PBMCs) and CD4+ T cells. In anti-CD3 stimulated PBMCs, GABA (100nM) inhibited release of 47 cytokines in cells from patients with type 1 diabetes (T1D), but only 16 cytokines in cells from nondiabetic (ND) individuals. CD4+ T cells from ND individuals were grouped into responder or non-responder T cells according to effects of GABA (100nM, 500nM) on the cell proliferation. In the responder T cells, GABA decreased proliferation, and inhibited secretion of 37 cytokines in a concentration-dependent manner. In the non-responder T cells, GABA modulated release of 8 cytokines. GABA concentrations in plasma from T1D patients and ND individuals were correlated with 10 cytokines where 7 were increased in plasma of T1D patients. GABA inhibited secretion of 5 of these cytokines from both T1D PBMCs and ND responder T cells. The results identify GABA as a potent regulator of both Th1- and Th2-type cytokine secretion from human PBMCs and CD4+ T cells where GABA generally decreases the secretion.

Place, publisher, year, edition, pages
2018. Vol. 30, p. 283-294
Keywords [en]
PBMCs, Immune cells, Proliferation, Cytokine, GABAA receptor, Diabetes, T1D, Autoimmune disease, T cell
National Category
Other Medical Sciences not elsewhere specified Endocrinology and Diabetes
Research subject
Biology; Physiology
Identifiers
URN: urn:nbn:se:uu:diva-348232DOI: 10.1016/j.ebiom.2018.03.019ISI: 000430303000033OAI: oai:DiVA.org:uu-348232DiVA, id: diva2:1196935
Funder
Swedish Research Council, 2015-02417Swedish Diabetes AssociationSwedish Child Diabetes FoundationEXODIAB - Excellence of Diabetes Research in SwedenAvailable from: 2018-04-11 Created: 2018-04-11 Last updated: 2018-06-19Bibliographically approved

Open Access in DiVA

GABA Regulates Release of Inflammatory Cytokines From PBMCs & CD4+ T Cells... (2018)(4374 kB)98 downloads
File information
File name FULLTEXT01.pdfFile size 4374 kBChecksum SHA-512
4fbeb3a8edc3267a06ad6424d67b8ab73d0db78296e8ce304f34fbf2e5b9edb4f1f873ef55e6332559930c16af58d695c956a0d09c9617760f0cc84871770d48
Type fulltextMimetype application/pdf

Other links

Publisher's full text

Authority records BETA

Bhandage, Amol K.Jin, ZheKorol, Sergiy V.Shen, QiujinEspes, DanielCarlsson, Per-OlaKamali-Moghaddam, MasoodBirnir, Bryndis

Search in DiVA

By author/editor
Bhandage, Amol K.Jin, ZheKorol, Sergiy V.Shen, QiujinEspes, DanielCarlsson, Per-OlaKamali-Moghaddam, MasoodBirnir, Bryndis
By organisation
PhysiologyScience for Life Laboratory, SciLifeLabMolecular toolsDepartment of Medical Cell BiologyTransplantation and regenerative medicine
In the same journal
EBioMedicine
Other Medical Sciences not elsewhere specifiedEndocrinology and Diabetes

Search outside of DiVA

GoogleGoogle Scholar
Total: 98 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

doi
urn-nbn

Altmetric score

doi
urn-nbn
Total: 497 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf