uu.seUppsala University Publications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Phylogenetic position and taxonomy of Kusaghiporia usambarensis gen. et sp. nov. (Polyporales)
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Organismal Biology, Systematic Biology. (Tibell)ORCID iD: 0000-0003-3442-9262
University of Dar es Salaam, Department of molecular Biology and Biotechnology.ORCID iD: 0000-0002-3105-8374
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Organismal Biology, Systematic Biology.ORCID iD: 0000-0003-4143-9856
2018 (English)In: Mycology, ISSN 2150-1203Article in journal (Refereed) Published
Abstract [en]

A large polyporoid mushroom from the West Usambara Mountains in North-eastern Tanzania produces dark brown, up to 60-cm large fruiting bodies that at maturity may weigh more than 10 kg. It has a high rate of mycelial growth and regeneration and was found growing on both dry and green leaves of shrubs; attached to the base of living trees, and it was also observed to degrade dead snakes and insects accidentally coming into contact with it. Phylogenetic analyses based on individual and concatenated data sets of nrLSU, nrSSU and the RPB2 and TEF1 genes showed it, together with Laetiporus, Phaeolus, Pycnoporellus and Wolfiporia, to form a monophyletic group in Polyporales. Based on morphological features and molecular data, it is described as Kusaghiporia usambarensis.

Place, publisher, year, edition, pages
Taylor & Francis, 2018.
Keywords [en]
Kusaghiporia; molecular phylogeny; polyporales; Tanzania; taxonomy; Usambara
National Category
Bioinformatics and Systems Biology
Research subject
Biology with specialization in Systematics
Identifiers
URN: urn:nbn:se:uu:diva-348537DOI: 10.1080/21501203.2018.1461142OAI: oai:DiVA.org:uu-348537DiVA, id: diva2:1197882
Funder
Sida - Swedish International Development Cooperation Agency, 2221Available from: 2018-04-15 Created: 2018-04-15 Last updated: 2020-02-28Bibliographically approved
In thesis
1. Polyporoid fungi of Tanzania: Taxonomy, transcriptomics and biochemical analyses of Kusaghiporia usambarensis and Piptoporellus baudonii
Open this publication in new window or tab >>Polyporoid fungi of Tanzania: Taxonomy, transcriptomics and biochemical analyses of Kusaghiporia usambarensis and Piptoporellus baudonii
2020 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Polyporoid fungi refers to basidiomycetes with fruiting bodies with the hymenium located to the inner surfaces of pores or narrow tubes. The majority of polyporoids belongs to Polyporales. Most Polyporales are saprobes, but some are plant pathogens. The overall aim of this thesis was to study the taxonomy, systematics and chemistry of the two species Kusaghiporia usambarensis (saprobic) and Piptoporellus baudonii (a plant pathogen) collected from Tanzania, using morphological and molecular approaches, combined with transcriptomics and pharmacognostic investigations.

The main contribution of this thesis includes the description a new genus with the new species K. usambarensis from the Usambara Mountains, Tanzania; investigation of the chemical composition of volatile compounds from this medicinal mushroom; isolation and structure determination of a novel and most abundant peptide in K. usambarensis, and further to elucidate the phylogenetic position of Piptoporellus baudonii (formerly known as Laetiporus baudonii) by using a four molecular markers dataset.

Paper I was conducted applying a classical taxonomic approach, including both morphological and phylogenetic analyses, to describe a new genus and species K. usambarensis. Paper II, investigated volatiles and volatile derivatives in dichloromethane extracts of K. usambarensis analysed by GC-MS and NMR spectroscopy. The main elements were phenols, and esters, compounds that may explain the formerly reported antioxidant activity and traditional medicinal use of the mushroom. In paper III, screening of peptides in K. usambarensis revealed a novel cysteine-rich peptide, highly expressed at gene level and the most abundant compound in the fruiting body. Combined LC-MS and transcriptome analyses were used to determine the peptide sequence, and subsequently NMR spectroscopy to determine the 3D structure of the novel peptide, kusaghitide. In paper IV molecular techniques were used to elucidate the phylogenetic position of the parasitic Laetiporus baudonii. Phylogenetic analyses of combined 5.8S, nrLSU, nrSSU, and TEF1 gene sequences placed L. baudonii in the genus Piptoporellus, hence the new combination Piptoporellus baudonii was proposed. This thesis has contributed to build capacity in the fields of mycology, systematics and pharmacognosy in order to reinforce ecological knowledge and ethnopharmaceutical research for future drug discovery in Tanzania and Africa at large.

Place, publisher, year, edition, pages
Uppsala: Acta Universitatis Upsaliensis, 2020. p. 56
Series
Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Science and Technology, ISSN 1651-6214 ; 1908
Keywords
Systematics, Laetiporaceae, pharmacognosy, cysteine-rich peptide, baudonii
National Category
Biological Sciences
Research subject
Biology with specialization in Systematics
Identifiers
urn:nbn:se:uu:diva-405548 (URN)978-91-513-0881-4 (ISBN)
Public defence
2020-04-17, Ekmansalen, Evolutionsbiologiskt centrum (EBC), Norbyvägen 14-18, Uppsala, 10:00 (English)
Opponent
Supervisors
Available from: 2020-03-26 Created: 2020-02-28 Last updated: 2020-03-26

Open Access in DiVA

fulltext(2466 kB)140 downloads
File information
File name FULLTEXT01.pdfFile size 2466 kBChecksum SHA-512
44162c42d74253dba596f8e8f169c477c4c9aea1954b2730d70698c07efc2c70051620eae32db10ef26d4864379361c84b7dfd34cef4de2e1af9d5f09958b59a
Type fulltextMimetype application/pdf

Other links

Publisher's full text

Authority records BETA

Hussein, JumaTibell, Sanja

Search in DiVA

By author/editor
Hussein, JumaTibuhwa, Donatha DamianTibell, Sanja
By organisation
Systematic Biology
Bioinformatics and Systems Biology

Search outside of DiVA

GoogleGoogle Scholar
Total: 140 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

doi
urn-nbn

Altmetric score

doi
urn-nbn
Total: 133 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf