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Engineered cyanobacteria with enhanced growth show increased ethanol production and higher biofuel to biomass ratio
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - Ångström, Molecular Biomimetics.
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - Ångström, Molecular Biomimetics.
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - Ångström, Molecular Biomimetics.ORCID iD: 0000-0002-6413-1443
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - Ångström, Molecular Biomimetics.
2018 (English)In: Metabolic engineering, ISSN 1096-7176, E-ISSN 1096-7184, Vol. 46, p. 51-59Article in journal (Refereed) Published
Abstract [en]

The Calvin-Benson-Bassham (CBB) cycle is the main pathway to fix atmospheric CO2 and store energy in carbon bonds, forming the precursors of most primary and secondary metabolites necessary for life. Speeding up the CBB cycle theoretically has positive effects on the subsequent growth and/or the end metabolite(s) production. Four CBB cycle enzymes, ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO), fructose-1,6/sedoheptulose-1,7-bisphosphatase (FBP/SBPase), transketolase (TK) and aldolase (FBA) were selected to be co-overexpressed with the ethanol synthesis enzymes pyruvate decarboxylase (PDC) and alcohol dehydrogenase (ADH) in the cyanobacterium Synechocystis PCC 6803. An inducible promoter, PnrsB, was used to drive PDC and ADH expression. When PnrsB was induced and cells were cultivated at 65 µmol photons m−2 s−1, the RuBisCO-, FBP/SBPase-, TK-, and FBA-expressing strains produced 55%, 67%, 37% and 69% more ethanol and 7.7%, 15.1%, 8.8% and 10.1% more total biomass (the sum of dry cell weight and ethanol), respectively, compared to the strain only expressing the ethanol biosynthesis pathway. The ethanol to total biomass ratio was also increased in CBB cycle enzymes overexpressing strains. This study experimentally demonstrates that using the cells with enhanced carbon fixation, when the product synthesis pathway is not the main bottleneck, can significantly increase the generation of a product (exemplified with ethanol), which acts as a carbon sink.

Place, publisher, year, edition, pages
2018. Vol. 46, p. 51-59
Keywords [en]
Cyanobacteria, Biofuel, Carbon fixation, Ethanol, RuBisCO, FBP/SBPase, TK, FBA
National Category
Biochemistry and Molecular Biology
Identifiers
URN: urn:nbn:se:uu:diva-351092DOI: 10.1016/j.ymben.2018.02.006ISI: 000427934100007PubMedID: 29477858OAI: oai:DiVA.org:uu-351092DiVA, id: diva2:1208727
Funder
Swedish Energy AgencyAvailable from: 2018-05-18 Created: 2018-05-18 Last updated: 2018-05-18Bibliographically approved

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Liang, FeiyanEnglund, EliasLindberg, PiaLindblad, Peter

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