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Production of hyaluronic acid-acrylamide microgels as potential cell culture scaffolds
National University of Singapore. (Exploring Microtechnology for Biomedical and Life science Applications)ORCID iD: 0000-0001-9102-956X
Uppsala University, Science for Life Laboratory, SciLifeLab. Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology. (Exploring Microtechnology for Biomedical and Life science Applications)ORCID iD: 0000-0001-5952-2418
Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology. Uppsala University, Science for Life Laboratory, SciLifeLab. (Exploring Microtechnology for Biomedical and Life science Applications)ORCID iD: 0000-0003-4475-6478
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - Ångström, Polymer Chemistry.ORCID iD: 0000-0002-2422-7280
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2018 (English)In: Micronano System Workshop, May 13-15, 2018: Book of Abstracts, 2018, p. 24-24Conference paper, Oral presentation with published abstract (Refereed)
Abstract [en]

Hyaluronic acid (HA) derived hydrogels give support and structure for cultured cells in 3D environments that better mimic in vivo conditions 1. Adequate diffusion of oxygen and nutrients however, is generally limited to a depth of 200 µm in bulk hydrogels 2, limiting their applicability to larger size constructs. Through droplet-based microfluidics we produced monodisperse HA-derived microgel droplets. Hyaluronic acid acrylamide (HA-am) was synthesized by partially modifying high molecular weight sodium hyaluronan with a N-(2-aminoethyl)acrylamide linker to a 20% degree.

Gel droplets were produced in a PDMS microfluidic device designed in a flow focusing geometry. In this setup polystyrene beads were added to simulate cell-encapsulation into a matrix that would better reflect in vivo conditions. The hydrogel precursor mixtures were prepared with 2% solution of HA-am and a photoinitiator with the addition of polystyrene beads (10µm in diameter) at a concentration of 10 million beads per milliliter. A fluorinated oil (Novec 7500, 3M) with 0.5% surfactant (PicoSurf 1) was used as the continuous phase. Highly monodisperse droplets of 151 µm in average diameter were produced and later polymerized by exposing to a long-wave UV light source (365 nm).  

We demonstrate that photocrosslinkable hydrogel droplets can be produced from HA-am. These microgels could enable the diffusion of nutrients and metabolites, while maintaining a size in which encapsulating sufficient cells to allow cell-cell interactions and proliferation would be possible.

[1]         J. A. Burdick and G. D. Prestwich, Adv. Mater., 2011, 23, 41–56.

[2]         H. Huang, Y. Yu, Y. Hu, X. He, O. Berk Usta and M. L. Yarmush, Lab Chip, 2017, 17, 1913–1932.

Place, publisher, year, edition, pages
2018. p. 24-24
National Category
Engineering and Technology
Research subject
Engineering Science with specialization in Microsystems Technology
Identifiers
URN: urn:nbn:se:uu:diva-357608OAI: oai:DiVA.org:uu-357608DiVA, id: diva2:1239828
Conference
Micronano System Workshop, Aalto University, Espoo, Finland, May 13th to 18th, 2018.
Available from: 2018-08-17 Created: 2018-08-17 Last updated: 2018-10-19Bibliographically approved

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Book of Abstracts MSW 2018

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Barbe, LaurentShi, LiyangPohlit, HannahTenje, Maria

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