uu.seUppsala University Publications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Transcriptional accuracy modeling suggests two-step proofreading by RNA polymerase
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Cell and Molecular Biology.
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Cell and Molecular Biology, Molecular Biology.
2017 (English)In: Nucleic Acids Research, ISSN 0305-1048, E-ISSN 1362-4962, Vol. 45, no 20, p. 11582-11593Article in journal (Refereed) Published
Abstract [en]

We suggest a novel two-step proofreading mechanism with two sequential rounds of proofreading selection in mRNA transcription. It is based on the previous experimental observations that the proofreading RNA polymerase cleaves off transcript fragments of at least 2 nt and that transcript elongation after a nucleotide misincorporation is anomalously slow. Taking these results into account, we extend the description of the accuracy of template guided nucleotide selection beyond previous models of RNA polymerase-dependent DNA transcription. The model derives the accuracy of initial and proofreading base selection from experimentally estimated nearest-neighbor parameters. It is also used to estimate the small accuracy enhancement of polymerase revisiting of previous positions following transcript cleavage.

Place, publisher, year, edition, pages
2017. Vol. 45, no 20, p. 11582-11593
National Category
Biochemistry and Molecular Biology
Identifiers
URN: urn:nbn:se:uu:diva-359630DOI: 10.1093/nar/gkx849ISI: 000416164300016PubMedID: 29036494OAI: oai:DiVA.org:uu-359630DiVA, id: diva2:1245427
Funder
Swedish Research Council, 2015-04682Knut and Alice Wallenberg Foundation, KAW 2009.0251Available from: 2018-09-05 Created: 2018-09-05 Last updated: 2018-09-05Bibliographically approved

Open Access in DiVA

fulltext(1097 kB)12 downloads
File information
File name FULLTEXT01.pdfFile size 1097 kBChecksum SHA-512
d22a3beb15557ca6d706383a8ee65e872f1d2daa96a4c7f0601228f2d8f71d7eae2cd2a467cc9740d3572b5ac49dc0958f6981708ceb259c622a400c84664361
Type fulltextMimetype application/pdf

Other links

Publisher's full textPubMed

Authority records BETA

Mellenius, HarrietEhrenberg, Måns

Search in DiVA

By author/editor
Mellenius, HarrietEhrenberg, Måns
By organisation
Department of Cell and Molecular BiologyMolecular Biology
In the same journal
Nucleic Acids Research
Biochemistry and Molecular Biology

Search outside of DiVA

GoogleGoogle Scholar
Total: 12 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

doi
pubmed
urn-nbn

Altmetric score

doi
pubmed
urn-nbn
Total: 51 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf