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Muscle RING-finger protein-1 (MuRF1) functions and cellular localization are regulated by SUMO1 post-translational modification
Department of Physiology and Pharmacology, Karolinska Institutet, Stockholm, Sweden.
Department of Physiology and Pharmacology, Karolinska Institutet, Stockholm, Sweden.
Department of Physiology and Pharmacology, Karolinska Institutet, Stockholm, Sweden.
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - BMC, Analytical Chemistry. (Mass spectrometry-based proteomics)
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2019 (English)In: Journal of Molecular Cell Biology, ISSN 1674-2788, E-ISSN 1759-4685, Vol. 11, no 5, p. 356-370Article in journal (Refereed) Published
Abstract [en]

The muscle RING-finger protein-1 (MuRF1) is an E3 ubiquitin ligase expressed in skeletal and cardiac muscle tissues and it plays important roles in muscle remodeling. Upregulation of MuRF1 gene transcription participates in skeletal muscle atrophy, on contrary downregulation of protein expression leads to cardiac hypertrophy. MuRF1 gene point mutations have been found to generate protein aggregate myopathies defined as muscle disorder characterized by protein accumulation in muscle fibers. We have discovered that MuRF1 turned out to be also a target for a new post-translational modification arbitrated by conjugation of SUMO1 and it is mediated by the SUMO ligases E2 UBC9 and the E3 PIASγ/4. SUMOylation takes place at lysine 238 localized at the second coiled-coil protein domain that is required for efficient substrate interaction for polyubiquitination. We provided evidence that SUMOylation is essential for MuRF1 nuclear translocation and its mitochondria accumulation is enhanced in hyperglycemic conditions delivering a stabilization of the overall SUMOylated proteins in cultured myocytes. Thus, our findings add this SUMO1 post-translational modification as a new concept to understand muscle disorders related to the defect in MuRF1 activity.

Place, publisher, year, edition, pages
2019. Vol. 11, no 5, p. 356-370
Keywords [en]
TRIM 63 /MuRF 1, muscle remodeling, SUMO, protein degradation, hyperglycemia
National Category
Physiology
Identifiers
URN: urn:nbn:se:uu:diva-361135DOI: 10.1093/jmcb/mjy036ISI: 000482800300003PubMedID: 29868881OAI: oai:DiVA.org:uu-361135DiVA, id: diva2:1250026
Funder
Swedish Research Council, 20133074Åke Wiberg Foundation, M14-0127Magnus Bergvall Foundation, 2015-01200Magnus Bergvall Foundation, 2016-01675Carl Tryggers foundation , CST 15:57Available from: 2018-09-21 Created: 2018-09-21 Last updated: 2019-10-02Bibliographically approved

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Shevchenko, GannaFalk, AlexanderBergström Lind, SaraMi, Jia

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