uu.seUppsala universitets publikationer
Ändra sökning
RefereraExporteraLänk till posten
Permanent länk

Direktlänk
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association
  • vancouver
  • Annat format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annat språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf
A novel, fast and sensitive supercritical fluid chromatography-tandem mass spectrometry (SFC-MS/MS) method for analysis of arachidonic acid metabolites
Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Kemiska sektionen, Institutionen för kemi - BMC, Analytisk kemi.ORCID-id: 0000-0002-5722-4908
Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Kemiska sektionen, Institutionen för kemi - BMC, Analytisk kemi.
Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Kemiska sektionen, Institutionen för kemi - BMC, Analytisk kemi.ORCID-id: 0000-0002-4597-041x
2018 (Engelska)Ingår i: The Analyst, ISSN 0003-2654, E-ISSN 1364-5528, Vol. 143, nr 15, s. 3661-3669Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

The development of a rapid, sensitive and reliable method for the quantification of bioactive arachidonic acid metabolites (AA-metabolites) in biological samples is quite challenging due to the minute concentration, short half-life and their structural complexity arising from different isomers. In this study, a simple, fast and environmentally friendly supercritical fluid chromatography-tandem mass spectrometry (SFC-MS/MS) method was developed and validated for simultaneous measurement of five (PGD(2), PGE(2), PGF(2), 6KetoPGF(1) and LTB4) AA-metabolites in biological samples. These analytes were extracted by protein precipitation followed by separation and quantification. The analysis was completed within 3 minutes. The matrix matched linear calibration ranged from 0.5-100 ng mL(-1) (r(2) 0.995), whilst, the limit of quantification of PGD(2), PGE(2), PGF(2), and LTB4 was 0.5 ng mL(-1) and was 2.5 ng mL(-1) for 6KetoPGF(1). The interday and intraday precisions of the method were less than 15% while the accuracy of most of the analytes varied between 83 and 109%. Finally, as a proof of concept, the method was successfully applied for the determination of eicosanoids in human samples, which expands the possibility to explore physiological states, disease phenotypes, and novel biomarkers.

Ort, förlag, år, upplaga, sidor
ROYAL SOC CHEMISTRY , 2018. Vol. 143, nr 15, s. 3661-3669
Nationell ämneskategori
Analytisk kemi
Identifikatorer
URN: urn:nbn:se:uu:diva-361490DOI: 10.1039/c8an00788hISI: 000439586400019PubMedID: 29971278OAI: oai:DiVA.org:uu-361490DiVA, id: diva2:1251545
Forskningsfinansiär
Vetenskapsrådet, 2015-4870Tillgänglig från: 2018-09-27 Skapad: 2018-09-27 Senast uppdaterad: 2018-09-27Bibliografiskt granskad

Open Access i DiVA

Fulltext saknas i DiVA

Övriga länkar

Förlagets fulltextPubMed

Personposter BETA

Ubhayasekera, KumariBergquist, Jonas

Sök vidare i DiVA

Av författaren/redaktören
Ubhayasekera, KumariBergquist, Jonas
Av organisationen
Analytisk kemi
I samma tidskrift
The Analyst
Analytisk kemi

Sök vidare utanför DiVA

GoogleGoogle Scholar

doi
pubmed
urn-nbn

Altmetricpoäng

doi
pubmed
urn-nbn
Totalt: 42 träffar
RefereraExporteraLänk till posten
Permanent länk

Direktlänk
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association
  • vancouver
  • Annat format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annat språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf