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Using standard additions to improve extraction and quantification of inositol hexakisphosphate in sediment samples by ion chromatography electrospray ionization mass spectrometry
Leibniz Inst Balt Sea Res Warnemuende, Dept Marine Chem, Seestr 15, D-18119 Rostock, Germany.
Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Kemiska sektionen, Institutionen för kemi - BMC.
Leibniz Inst Balt Sea Res Warnemuende, Dept Marine Chem, Seestr 15, D-18119 Rostock, Germany.
Leibniz Inst Balt Sea Res Warnemuende, Dept Marine Chem, Seestr 15, D-18119 Rostock, Germany.
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2018 (Engelska)Ingår i: Talanta: The International Journal of Pure and Applied Analytical Chemistry, ISSN 0039-9140, E-ISSN 1873-3573, Vol. 188, s. 192-198Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Several key aspects for the analysis of inositol hexakisphosphate (InsP(6)) have been investigated in order to establish a suitable method for the study of sediment samples from different aquatic systems. Apparent matrix effects for the ion chromatography electrospray ionization tandem mass spectrometric detection (IC-ESI-MS/MS) method were accounted for with a standard addition approach, which also compensated for variation in extraction efficiency. Several parameters of the extraction method were optimized to improve the extraction efficiency for different sediment types. We observed an improvement in the extraction efficiency between 18% and 720%. Finally, the method was used to gain first insights into the relevance of InsP(6) in two aquatic systems located at the German Baltic coastal area. InsP(6) was detected in several sediment samples with concentrations between 2.3 and 15.2 mu g InsP(6)-P/g dry weight (DW).

Ort, förlag, år, upplaga, sidor
2018. Vol. 188, s. 192-198
Nyckelord [en]
Inositol phosphates, IC-ESI-MS, Standard addition, Sediment
Nationell ämneskategori
Analytisk kemi
Identifikatorer
URN: urn:nbn:se:uu:diva-362627DOI: 10.1016/j.talanta.2018.05.072ISI: 000440958700024PubMedID: 30029363OAI: oai:DiVA.org:uu-362627DiVA, id: diva2:1254759
Forskningsfinansiär
EU, Horisont 2020Tillgänglig från: 2018-10-10 Skapad: 2018-10-10 Senast uppdaterad: 2018-10-10Bibliografiskt granskad

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Sjöberg, Per J. R.

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Talanta: The International Journal of Pure and Applied Analytical Chemistry
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