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Characterization of a cold-active transglutaminase from a crayfish, Pacifastacus leniusculus
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Organismal Biology, Comparative Physiology.
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Organismal Biology, Comparative Physiology.
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Organismal Biology, Comparative Physiology.
2018 (English)In: Fish and Shellfish Immunology, ISSN 1050-4648, E-ISSN 1095-9947, Vol. 80, p. 546-549Article in journal (Refereed) Published
Abstract [en]

Transglutaminase (TGase) from signal crayfish (Pacifastacus leniusculus) and its activity at low temperatures was studied. TGase is an abundant protein in the hematopoietic (HPT) cells and this tissue was used for TGase enzyme preparation. The optimal temperature and pH for the activity of crayfish TGase were determined. We found that TGase activity at 4 degrees C showed nearly the same activity as at a temperature of 22 degrees C. TGase activity from crayfish was compared with guinea pig liver TGase activity at 4 degrees C and the crayfish TGase displayed a higher activity while guinea pig liver TGase had a very low activity at this low temperature. By comparing kinetic parameters to guinea pig liver TGase, the results showed that a high activity of crayfish TGase was due to a decreasing K-m value for pentylamine as a substrate, while it did not affect the k(cat) value (at 22 degrees C). The amino acid sequences of a krill and a crayfish TGase, which both are cold adapted, do not give any clue to why these two enzymes are cold-adapted. These results demonstrate that crayfish TGase is adapted to have significant activity at low temperatures and since crayfish are living in quite cold waters this is an interesting adaptation of this enzyme.

Place, publisher, year, edition, pages
2018. Vol. 80, p. 546-549
Keywords [en]
Cold-adapted enzyme, Crayfish, Transglutaminase
National Category
Immunology
Identifiers
URN: urn:nbn:se:uu:diva-363096DOI: 10.1016/j.fsi.2018.06.042ISI: 000440958100061PubMedID: 29960064OAI: oai:DiVA.org:uu-363096DiVA, id: diva2:1256316
Funder
Swedish Research Council, VR 621-2012-2418Swedish Research Council Formas, FORMAS 2011-606Available from: 2018-10-16 Created: 2018-10-16 Last updated: 2018-10-16Bibliographically approved

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Sirikharin, RatchanokSöderhäll, IreneSöderhäll, Kenneth

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