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Effect of cobalt ions on the interaction between macrophages and titanium
Umea Univ, Prosthet Dent, Umea, Sweden.ORCID iD: 0000-0001-5992-8135
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - BMC, Analytical Chemistry.
Umea Univ, Prosthet Dent, Umea, Sweden.
Umea Univ, Fac Med, Dept Odontol, Mol Periodontol, Umea, Sweden.
2018 (English)In: Journal of Biomedical Materials Research. Part A, ISSN 1549-3296, E-ISSN 1552-4965, Vol. 106, no 9, p. 2518-2530Article in journal (Refereed) Published
Abstract [en]

Inflammation and bone reduction around dental implants are described as peri-implantitis and can be caused by an inflammatory response against bacterial products and toxins. Titanium (Ti) forms aggregates with serum proteins, which activate and cause release of the cytokine interleukin (IL-1) from human macrophages. It was hypothesized that cobalt (Co) ions can interact in the formation of pro-inflammatory aggregates, formed by titanium. To test this hypothesis, we differentiated THP-1 cells into macrophages and exposed them to Ti ions alone or in combination with Co ions to investigate if IL-1 release and cytotoxicity were affected. We also investigated aggregate formation, cell uptake and human biopsies with inductively coupled plasma atomic emission spectroscopy and electron microscopy. Co at a concentration of 100 mu M neutralized the IL-1 release from human macrophages and affected the aggregate formation. The aggregates formed by Ti could be detected in the cytosol of macrophages. In the presence of Co, the Ti-induced aggregates were located in the cytosol of the cultured macrophages, but outside the lysosomal structures. It is concluded that Co can neutralize the Ti-induced activation and release of active IL-1 from human macrophages in vitro. Also, serum proteins are needed for the formation of metal-protein aggregates in cell medium. Furthermore, the structures of the aggregates as well as the localisation after cellular uptake differ if Co is present in a Ti solution. Phagocytized aggregates with a similar appearance seen in vitro with Ti present, were also visible in a sample from human peri-implant tissue.

Place, publisher, year, edition, pages
WILEY , 2018. Vol. 106, no 9, p. 2518-2530
Keywords [en]
titanium, cobalt, interleukin-1, peri-implantitis, aggregate formation
National Category
Biomaterials Science
Identifiers
URN: urn:nbn:se:uu:diva-363623DOI: 10.1002/jbm.a.36447ISI: 000445615600016PubMedID: 29708655OAI: oai:DiVA.org:uu-363623DiVA, id: diva2:1258835
Funder
Västerbotten County Council, VLL 1147-2014Available from: 2018-10-25 Created: 2018-10-25 Last updated: 2018-10-25Bibliographically approved

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Pettersson, Jean

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