uu.seUppsala University Publications
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Towards the use of Alcohol dehydrogenases as biocatalysts for stereoselective isotope labeling of aromatic alcohols.
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - BMC.
2018 (English)Independent thesis Basic level (degree of Bachelor), 10 credits / 15 HE creditsStudent thesis
Abstract [en]

The enzyme ADH-A and one of its mutants ADH-A C1B1 from ​ Rhodococcus ruber,​ have in previous studies been proved to act as proper biocatalysts, fully capable of performing redox reactions. Two redox reactions were studied during this project, were those enzymes act as catalysts. For that matter, ADH-A wild type and ADH-A C1B1 genes were expressed in ​ E. coli​ and the encoded enzymes were purified and used for kinetic studies with a final goal on studying the kinetic isotope effect that is generated between them and the molecules that contain deuterium. HPLC analysis on these products showed that the reactions were not thermodynamically favored and conclusions on the best reaction conditions for both enzymes as well as for further improvements are discussed.

Place, publisher, year, edition, pages
2018. , p. 29
Series
UPKEM C ; 128
National Category
Chemical Sciences
Identifiers
URN: urn:nbn:se:uu:diva-368315OAI: oai:DiVA.org:uu-368315DiVA, id: diva2:1267850
Subject / course
Chemistry
Educational program
Bachelor Programme in Chemistry
Presentation
2018-10-22, C8:321, Husargatan 3, 752 37, Uppsala, 09:41 (English)
Supervisors
Examiners
Available from: 2018-12-05 Created: 2018-12-04 Last updated: 2018-12-05Bibliographically approved

Open Access in DiVA

fulltext(1326 kB)2 downloads
File information
File name FULLTEXT01.pdfFile size 1326 kBChecksum SHA-512
8dfbba69dca569261eb27e96a69cee1c0c1a18ab9f32b237e2b89ddcdf4049eb4ed0aab69809ef7dbcee0a0f9c144a4d1dec81b803573a021d750ff8947cd21e
Type fulltextMimetype application/pdf

By organisation
Department of Chemistry - BMC
Chemical Sciences

Search outside of DiVA

GoogleGoogle Scholar
Total: 2 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

urn-nbn

Altmetric score

urn-nbn
Total: 3 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf