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Working Towards the Heterologous Expression of Styrene Monooxygenases for Styrene Epoxidation and Reaction Cascades
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - BMC.
2018 (English)Independent thesis Basic level (degree of Bachelor), 10 credits / 15 HE creditsStudent thesis
Abstract [en]

Previously, styrene oxide has been used as starting material in the reaction cascade for obtaining 2-hydroxyacetophenone. If the path could be extended to instead start with styrene as substrate, it would be an advantage financially. The aim of this degree project was to produce two monooxygenase components needed for the epoxidation of styrene. The coding sequence for styrene monooxygenase component StyA had in an earlier project been inserted in a plasmid which in this project was used for inserting the gene coding for the styrene monooxygenase component StyB. The transformation of the ligated plasmid became problematic and did not result in the expected outcome. When doing an transformation directly on the ligation mixture, the result of the experiment was successful. Consequently, the problem was likely due to the poor condition of the cells used.

Place, publisher, year, edition, pages
2018. , p. 19
Series
UPKEM C ; 127
National Category
Chemical Sciences
Identifiers
URN: urn:nbn:se:uu:diva-368314OAI: oai:DiVA.org:uu-368314DiVA, id: diva2:1267852
Subject / course
Chemistry
Educational program
Bachelor Programme in Chemistry
Presentation
2018-10-22, C8:321, Husargatan 3, Uppsala, 09:41 (English)
Supervisors
Examiners
Available from: 2018-12-05 Created: 2018-12-04 Last updated: 2018-12-05Bibliographically approved

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Chemical Sciences

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