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Differences in secretome in culture media when comparing blastocysts and arrested embryos using multiplex proximity assay
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Women's and Children's Health, Research group (Dept. of women´s and children´s health), Reproductive Health.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Women's and Children's Health.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Immunology, Genetics and Pathology. Karolinska Univ Hosp, Karolinska Inst, Dept Clin Sci Intervent & Technol, SE-14186 Stockholm, Sweden;Karolinska Univ Hosp, Unit Reprod Med, SE-14186 Stockholm, Sweden.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Women's and Children's Health, Research group (Dept. of women´s and children´s health), Reproductive biology. Carl von Linne Clin, SE-75183 Uppsala, Sweden.
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2018 (English)In: Upsala Journal of Medical Sciences, ISSN 0300-9734, E-ISSN 2000-1967, Vol. 123, no 3, p. 143-152Article in journal (Refereed) Published
Abstract [en]

Objectives: The aim of this study was to assess different patterns of the human embryo secretome analysed as protein levels in culture media. Furthermore, analyses to correlate protein levels with quality and timing to development of human embryos were performed.

Material and methods: Human day-2 cryopreserved embryos were cultured for four days in an EmbryoScope((R)) with a time-lapse camera, and embryo quality was evaluated retrospectively. After culture, the media were collected and relative levels of secreted proteins were analysed using Proseek Multiplex Assays. Protein levels were evaluated in relation to timing to development and the ability to form a blastocyst.

Results: Specific patterns of timing of development of blastocysts were found, where a difference in time to start of cavitation was found between high- and low-quality blastocysts. There appeared to be a correlation between specific protein patterns and successful formation of morulae and blastocysts. Embryos developing into blastocysts had higher levels of EMMPRIN than arrested embryos, and levels of caspase-3 were lower in high- versus low-quality blastocysts. Also, higher levels of VEGF-A, IL-6, and EMMPRIN correlated with shorter times to morula formation.

Conclusions: The secretome and timing to development differ in embryos forming blastocysts and those that become arrested, and in high- versus low-quality blastocysts. The levels of certain proteins also correlate to specific times to development.

Place, publisher, year, edition, pages
TAYLOR & FRANCIS LTD , 2018. Vol. 123, no 3, p. 143-152
Keywords [en]
Blastocyst, caspase-3, extracellular matrix metalloproteinase inducer, interleukin-6, prediction, secretome, time-lapse, vascular endothelial growth factor A
National Category
Obstetrics, Gynecology and Reproductive Medicine
Identifiers
URN: urn:nbn:se:uu:diva-368773DOI: 10.1080/03009734.2018.1490830ISI: 000446977000002PubMedID: 30282508OAI: oai:DiVA.org:uu-368773DiVA, id: diva2:1269458
Funder
Swedish Research Council, FF-2017-477Swedish Society of MedicineStiftelsen Olle Engkvist ByggmästareAvailable from: 2018-12-10 Created: 2018-12-10 Last updated: 2018-12-10Bibliographically approved

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Lindgren, Karin ElvineHreinsson, JuliusHolte, JanKårehed, KarinSundström Poromaa, IngerKaihola, HelenaÅkerud, Helena

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Lindgren, Karin ElvineHreinsson, JuliusHolte, JanKårehed, KarinSundström Poromaa, IngerKaihola, HelenaÅkerud, Helena
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Reproductive HealthDepartment of Women's and Children's HealthDepartment of Immunology, Genetics and PathologyReproductive biologyObstetrics and Reproductive Health ResearchMedicinsk genetik och genomik
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Obstetrics, Gynecology and Reproductive Medicine

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