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In vivo efficiency of antimicrobial inorganic bone grafts in osteomyelitis Treatments
Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology. Uppsala University, Science for Life Laboratory, SciLifeLab. (EMBLA/MST)ORCID iD: 0000-0001-7462-4236
Technical University of Catalonia (UPC). (Biomaterials, Biomechanics and Tissue Engineering Group, Dpt. Materials Science and Metallurgy)
Technical University of Catalonia.
Brno University of Technology.
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2019 (English)In: Materials science & engineering. C, biomimetic materials, sensors and systems, ISSN 0928-4931, E-ISSN 1873-0191, Vol. 97, p. 84-95Article in journal (Refereed) Published
Abstract [en]

The purpose of the present work was to evaluate in vivo different antimicrobial therapies to eradicate osteomyelitis created in the femoral head of New Zealand rabbits. Five phosphate-based cements were evaluated: calcium phosphate cements (CPC) and calcium phosphate foams (CPF), both in their pristine form and loaded with doxycycline hyclate, and an intrinsic antimicrobial magnesium phosphate cement (MPC; not loaded with an antibiotic). The cements were implanted in a bone previously infected with Staphylococcus aureus to discern the effects of the type of antibiotic administration (systemic vs. local), porosity (microporosity, i.e. <5 μm vs. macroporosity, i.e. >5 μm) and type of antimicrobial mechanism (release of antibiotic vs. intrinsic antimicrobial activity) on the improvement of the health state of the infected animals. A new method was developed, with a more comprehensive composite score that integrates 5 parameters of bone infection, 4 parameters of bone structural integrity and 4 parameters of bone regeneration. This method was used to evaluate the health state of the infected animals, both before and after osteomyelitis treatment. The results showed that the composite score allows to discern statistically significant differences between treatments that individual evaluations were not able to identify. Despite none of the therapies completely eradicated the infection, it was observed that macroporous materials (CPF and CPFd, the latter loaded with doxycycline hyclate) and intrinsic antimicrobial MPC allowed a better containment of the osteomyelitis. This study provides novel insights to understand the effect of different antimicrobial therapies in vivo, and a promising comprehensive methodology to evaluate the health state of the animals was developed. We expect that the implementation of such methodology could improve the criteria to select a proper antimicrobial therapy.

Place, publisher, year, edition, pages
2019. Vol. 97, p. 84-95
Keywords [en]
Osteomyelitis; Calcium phosphate cements; Calcium phosphate foams; Magnesium phosphate cements; Drug delivery; In vivo
National Category
Biomaterials Science
Research subject
Engineering Science with specialization in Materials Science
Identifiers
URN: urn:nbn:se:uu:diva-369961DOI: 10.1016/j.msec.2018.11.064ISI: 000457952800009PubMedID: 30678975OAI: oai:DiVA.org:uu-369961DiVA, id: diva2:1271659
Note

De 2 första författarna delar förstaförfattarskapet.

Available from: 2018-12-17 Created: 2018-12-17 Last updated: 2019-03-08Bibliographically approved

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The full text will be freely available from 2020-11-28 11:07
Available from 2020-11-28 11:07

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Mestres, Gemma

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