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Cryo-EM structure of a Marseilleviridae virus particle reveals a large internal microassembly.
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Cell and Molecular Biology, Molecular biophysics.
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Cell and Molecular Biology, Molecular biophysics.
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Cell and Molecular Biology, Molecular biophysics.
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2018 (English)In: Virology, ISSN 0042-6822, E-ISSN 1096-0341, Vol. 516, p. 239-245, article id S0042-6822(18)30028-XArticle in journal (Refereed) Published
Abstract [en]

Nucleocytoplasmic large DNA viruses (NCLDVs) blur the line between viruses and cells. Melbournevirus (MelV, family Marseilleviridae) belongs to a new family of NCLDVs. Here we present an electron cryo-microscopy structure of the MelV particle, with the large triangulation number T = 309 constructed by 3080 pseudo-hexagonal capsomers. The most distinct feature of the particle is a large and dense body (LDB) consistently found inside all particles. Electron cryo-tomography of 147 particles shows that the LDB is preferentially located in proximity to the probable lipid bilayer. The LDB is 30 nm in size and its density matches that of a genome/protein complex. The observed LDB reinforces the structural complexity of MelV, setting it apart from other NCLDVs.

Place, publisher, year, edition, pages
2018. Vol. 516, p. 239-245, article id S0042-6822(18)30028-X
Keywords [en]
Amoeba, Capsid, Cryo-electron microscopy, Marseilleviridae, Melbournevirus, NCLDV, Protein complex, Structure, Tomography, Virus
National Category
Biophysics
Identifiers
URN: urn:nbn:se:uu:diva-370071DOI: 10.1016/j.virol.2018.01.021PubMedID: 29407382OAI: oai:DiVA.org:uu-370071DiVA, id: diva2:1272303
Available from: 2018-12-18 Created: 2018-12-18 Last updated: 2019-01-24Bibliographically approved

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Okamoto, KentaReddy, Hemanth K.N.Maia, Filipe R.N.C.Larsson, Daniel S DHajdu, JSvenda, Martin

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