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Cryo-EM structure of a Marseilleviridae virus particle reveals a large internal microassembly
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Cell and Molecular Biology, Molecular biophysics.
National Institute for Physiological Sciences (NIPS), Okazaki, Aichi, 444-8585 Japan.
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Cell and Molecular Biology, Molecular biophysics.
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Cell and Molecular Biology, Molecular biophysics.
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2018 (English)In: Virology, ISSN 0042-6822, E-ISSN 1096-0341, Vol. 516, p. 239-245, article id S0042-6822(18)30028-XArticle in journal (Refereed) Published
Abstract [en]

Nucleocytoplasmic large DNA viruses (NCLDVs) blur the line between viruses and cells. Melbournevirus (MelV, family Marseilleviridae) belongs to a new family of NCLDVs. Here we present an electron cryo-microscopy structure of the MelV particle, with the large triangulation number T = 309 constructed by 3080 pseudo-hexagonal capsomers. The most distinct feature of the particle is a large and dense body (LDB) consistently found inside all particles. Electron cryo-tomography of 147 particles shows that the LDB is preferentially located in proximity to the probable lipid bilayer. The LDB is 30 nm in size and its density matches that of a genome/protein complex. The observed LDB reinforces the structural complexity of MelV, setting it apart from other NCLDVs.

Place, publisher, year, edition, pages
2018. Vol. 516, p. 239-245, article id S0042-6822(18)30028-X
Keywords [en]
Amoeba, Capsid, Cryo-electron microscopy, Marseilleviridae, Melbournevirus, NCLDV, Protein complex, Structure, Tomography, Virus
National Category
Biophysics
Identifiers
URN: urn:nbn:se:uu:diva-370071DOI: 10.1016/j.virol.2018.01.021ISI: 000428004800025PubMedID: 29407382OAI: oai:DiVA.org:uu-370071DiVA, id: diva2:1272303
Funder
Swedish Research Council, 628-20081109 822-2010-6157 822-2012-5260 828-2012-108Knut and Alice Wallenberg Foundation, KAW-2011.081EU, European Research Council, ERC-291602The Swedish Foundation for International Cooperation in Research and Higher Education (STINT), JA2014-5721Available from: 2018-12-18 Created: 2018-12-18 Last updated: 2019-08-25Bibliographically approved
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Okamoto, KentaReddy, Hemanth K.N.Maia, Filipe R.N.C.Larsson, Daniel S DHajdu, JanosSvenda, Martin

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