uu.seUppsala universitets publikasjoner
Endre søk
RefereraExporteraLink to record
Permanent link

Direct link
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association
  • vancouver
  • Annet format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annet språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf
Characteristics of the tissue section that influence the staining outcome in immunohistochemistry
Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för immunologi, genetik och patologi, Klinisk och experimentell patologi. Department of Pathology, Uppsala University Hospital, Uppsala, Sweden. (Irina Alafuzoff)
Department of Pathology, Uppsala University Hospital, Uppsala, Sweden.
Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för immunologi, genetik och patologi, Klinisk och experimentell patologi. Department of Pathology, Uppsala University Hospital, Uppsala, Sweden. (Irina Alafuzoff)
2019 (engelsk)Inngår i: Histochemistry and Cell Biology, ISSN 0948-6143, E-ISSN 1432-119X, Vol. 151, nr 1, s. 91-96Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

Immunohistochemistry (IHC) is influenced by several factors such as cold ischemia time, fixative, fixation time, paraffin, storage time, antibody, antigen retrieval technique and detection systems. In the setting of post-mortem tissue, not only post-mortem delay, but also agonal state is of interest. Here, we assessed an additional variable, i.e., the thickness of the section, and noted that this variable also influenced the IHC outcome. This is of significance when the extent of labelling is a parameter to be assessed, for example when assigning a stage or grade of a disease. Furthermore, when assessing brain tissue with neurons, soma measuring from 4 to 100 µm, various cellular compartments composed of different proteins are localised in sections measuring 4 or 7 µm. Thus, what is seen in a 7-µm-thick section might be lacking in a 4-µm-thick section. Lack of information regarding the molecular size of commercial antibodies is also disturbing as this parameter might influence the distribution of the molecule in the three-dimensional section. The choice of antibody to be used and the staining methodology have been acknowledged being of significance for IHC outcome; however, neither sections thickness or the molecular weight has been discussed sufficiently. IHC has been shown to be an unpredictable technique used for assessment of tissue. This emphasises the need for detailed methodological descriptions in publications, the need to acknowledge and to harmonize all eventual pitfalls related to this methodology.

sted, utgiver, år, opplag, sider
2019. Vol. 151, nr 1, s. 91-96
Emneord [en]
Extent of staining, Immunohistochemistry, Pitfalls, Thickness of a section
HSV kategori
Forskningsprogram
Patologi
Identifikatorer
URN: urn:nbn:se:uu:diva-372540DOI: 10.1007/s00418-018-1742-1ISI: 000455442800009PubMedID: 30357509OAI: oai:DiVA.org:uu-372540DiVA, id: diva2:1276043
Forskningsfinansiär
Hans-Gabriel och Alice Trolle-Wachtmeisters stiftelse för medicinsk forskningTilgjengelig fra: 2019-01-07 Laget: 2019-01-07 Sist oppdatert: 2019-03-29bibliografisk kontrollert

Open Access i DiVA

fulltext(1314 kB)34 nedlastinger
Filinformasjon
Fil FULLTEXT01.pdfFilstørrelse 1314 kBChecksum SHA-512
60e45eec7fb001255d626ab4462fbfab700193b74d6e30237b97d15a47881cd8961c4fe1eef10b523af97baace8b95448742f907ba827df3bc28513981fcf5a6
Type fulltextMimetype application/pdf

Andre lenker

Forlagets fulltekstPubMed

Personposter BETA

Libard, SylwiaCerjan, DijanaAlafuzoff, Irina

Søk i DiVA

Av forfatter/redaktør
Libard, SylwiaCerjan, DijanaAlafuzoff, Irina
Av organisasjonen
I samme tidsskrift
Histochemistry and Cell Biology

Søk utenfor DiVA

GoogleGoogle Scholar
Totalt: 34 nedlastinger
Antall nedlastinger er summen av alle nedlastinger av alle fulltekster. Det kan for eksempel være tidligere versjoner som er ikke lenger tilgjengelige

doi
pubmed
urn-nbn

Altmetric

doi
pubmed
urn-nbn
Totalt: 88 treff
RefereraExporteraLink to record
Permanent link

Direct link
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association
  • vancouver
  • Annet format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annet språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf