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A glassy carbon electrode modified with TiO2(200)-rGO hybrid nanosheets for aptamer based impedimetric determination of the prostate specific antigen
Vali E Asr Univ Rafsanjan, Dept Phys, Rafsanjan 7713936417, Iran.
Vali E Asr Univ Rafsanjan, Dept Chem, Fac Sci, Rafsanjan 7718897111, Iran.
Vali E Asr Univ Rafsanjan, Dept Phys, Rafsanjan 7713936417, Iran.
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - Ångström, Physical Chemistry.ORCID iD: 0000-0003-2046-1229
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2019 (English)In: Microchimica Acta, ISSN 0026-3672, E-ISSN 1436-5073, Vol. 186, no 1, article id 33Article in journal (Refereed) Published
Abstract [en]

TiO2(200)-rGO hybrid nanosheets were synthesized starting from TiO2, rGO and NaOH solid powders via a scalable hydrothermal process. The weight ratio of TiO2-GO was found to be crucial on the crystal growth and biosensor properties of the final hybrid nanosheets. They were characterized by means of SEM, FESEM-EDX, XRD, XPS, Raman and FTIR spectroscopies in order to verify the formation of very thin TiO2 anatase nanosheets with an orientation of the anatase crystal structure towards the (200) plane. The free active sites of TiO2 structure and the large surface of the 2D graphene structure strongly facilitate charge transport confirmed by BET-BJH analyses. Compared to pure AuNPs, rGO and TiO2, the hybrid nanosheet modified electrode represents the most sensitive aptasensing platform for the determination of PSA. The detection was based on that the variation of electron transfer resistance (Rct) at the modified electrode surface in a solution containing 3.0mmolL(-1) [Fe(CN)(6)](3-/4-) as a redox probe and 0.1molL(-1) KCl as supporting electrolyte. The detection limit of the sensor is 1pgmL(-1), and the sensor can be operated up to 30days. It was applied to the analysis of PSA levels in spiked serum samples obtained from patients with prostate cancer. Data compare well with those obtained by an immunoradiometric assay.

Place, publisher, year, edition, pages
SPRINGER WIEN , 2019. Vol. 186, no 1, article id 33
Keywords [en]
Work function, X-ray photoelectron spectroscopy, Effective surface area, Aptasensor, Electrochemical impedance spectroscopy, Voltammetry
National Category
Materials Chemistry
Identifiers
URN: urn:nbn:se:uu:diva-372873DOI: 10.1007/s00604-018-3141-7ISI: 000453796800007PubMedID: 30564911OAI: oai:DiVA.org:uu-372873DiVA, id: diva2:1277286
Available from: 2019-01-10 Created: 2019-01-10 Last updated: 2019-01-10Bibliographically approved

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Johansson, Malin B

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