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Single-Molecule Tracking Approaches to Protein Synthesis Kinetics in Living Cells
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Cell and Molecular Biology, Molecular Systems Biology.ORCID iD: 0000-0001-7288-6363
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Cell and Molecular Biology, Molecular Systems Biology.ORCID iD: 0000-0001-8811-2629
2019 (English)In: Biochemistry, ISSN 0006-2960, E-ISSN 1520-4995, Vol. 58, no 1, p. 7-14Article in journal (Refereed) Published
Abstract [en]

Decades of traditional biochemistry, structural approaches, and, more recently, single-molecule-based in vitro techniques have provided us with an astonishingly detailed understanding of the molecular mechanism of ribosome-catalyzed protein synthesis. However, in order to understand these details in the context of cell physiology and population biology, new techniques to probe the dynamics of molecular processes inside the cell are needed. Recent years' development in super-resolved fluorescence microscopy has revolutionized imaging of intracellular processes, and we now have the possibility to directly peek into the microcosm of biomolecules in their native environment. In this Perspective, we discuss how these methods are currently being applied and further developed to study the kinetics of protein synthesis directly inside living cells.

Place, publisher, year, edition, pages
AMER CHEMICAL SOC , 2019. Vol. 58, no 1, p. 7-14
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Biochemistry and Molecular Biology
Identifiers
URN: urn:nbn:se:uu:diva-375887DOI: 10.1021/acs.biochem.8b00917ISI: 000455557700002PubMedID: 30404437OAI: oai:DiVA.org:uu-375887DiVA, id: diva2:1285276
Funder
Swedish Research Council, 2015-04111Swedish Research Council, 2016-06264Wenner-Gren FoundationsCarl Tryggers foundation , 15:243Available from: 2019-02-04 Created: 2019-02-04 Last updated: 2019-02-04Bibliographically approved

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Volkov, IvanJohansson, Magnus

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