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Copper tolerance of the soft-rot fungus Phialophora malorum grown in-vitro revealed by microscopy and global protein expression
Swedish Univ Agr Sci, Dept Forest Prod Wood Sci, Box 7008, SE-75007 Uppsala, Sweden.
Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Kemiska sektionen, Institutionen för kemi - BMC, Analytisk kemi.ORCID-id: 0000-0003-4764-1246
Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Kemiska sektionen, Institutionen för kemi - BMC, Analytisk kemi.
Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Kemiska sektionen, Institutionen för kemi - BMC, Analytisk kemi.ORCID-id: 0000-0002-4597-041x
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2019 (engelsk)Inngår i: International Biodeterioration & Biodegradation, ISSN 0964-8305, E-ISSN 1879-0208, Vol. 137, s. 147-152Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

In this study, we used proteomics in conjunction with microscopy to study differences in the proteome and hyphal morphology of the copper tolerant soft rot fungus Phialophora malorum grown in media containing 0.064, 0.64% Cu as CuSO4. Unique proteins were found in the control and the copper-treated (0.064% CuSO4) samples. Of five unique proteins found in the 0.064% CuSO4 treated cultures, ATP synthase subunit alpha is considered to play an important role in copper tolerance as it is involved in the biosynthesis of fatty acids and steroids and may relate to morphological changes associated with hyphal cell walls of the fungus when grown in the presence of copper. ICP-AES analyses showed total mycelial Cu to increase with media Cu with 5246- and 16535 mu g Cu/g dry wt mycelia respectively found in 0.064 and 0.64% Cu-cultures after 6 weeks growth. Rubeanic acid staining of 0.064% mycelia showed Cu bound in intracellular bodies while most Cu was found as extracellular precipitates on the surfaces of hyphae in 0.64% Cu. SEM showed hyphal surfaces enrobed in fibrillar polysaccharides to which Cu was bound.

sted, utgiver, år, opplag, sider
2019. Vol. 137, s. 147-152
Emneord [en]
Copper tolerance, ICP-AES, LC-MS/MS, Phialophora malorum, Proteomics, Soft rot fungi
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Identifikatorer
URN: urn:nbn:se:uu:diva-378196DOI: 10.1016/j.ibiod.2018.12.001ISI: 000458470800017OAI: oai:DiVA.org:uu-378196DiVA, id: diva2:1294550
Forskningsfinansiär
Swedish Research Council Formas, 2011-416Swedish Research Council Formas, 2011-6383-19675Swedish Research Council Formas, 621-2015-4870Tilgjengelig fra: 2019-03-07 Laget: 2019-03-07 Sist oppdatert: 2019-03-07bibliografisk kontrollert

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