uu.seUppsala University Publications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Extended insight into the Mycobacterium chelonae-abscessus complex through whole genome sequencing of Mycobacterium salmoniphilum outbreak and Mycobacterium salmoniphilum-like strains
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Cell and Molecular Biology, Molecular Biology.ORCID iD: 0000-0002-8810-6066
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Cell and Molecular Biology, Molecular Biology.ORCID iD: 0000-0001-8799-691X
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Cell and Molecular Biology, Molecular Biology.ORCID iD: 0000-0003-4177-8145
Univ Louisiana, Dept Biol, Lafayette, LA USA.
Show others and affiliations
2019 (English)In: Scientific Reports, ISSN 2045-2322, E-ISSN 2045-2322, Vol. 9, article id 4603Article in journal (Refereed) Published
Abstract [en]

Members of the Mycobacterium chelonae-abscessus complex (MCAC) are close to the mycobacterial ancestor and includes both human, animal and fish pathogens. We present the genomes of 14 members of this complex: the complete genomes of Mycobacterium salmoniphilum and Mycobacterium chelonae type strains, seven M. salmoniphilum isolates, and five M. salmoniphilum-like strains including strains isolated during an outbreak in an animal facility at Uppsala University. Average nucleotide identity (ANI) analysis and core gene phylogeny revealed that the M. salmoniphilum-like strains are variants of the human pathogen Mycobacterium franklinii and phylogenetically close to Mycobacterium abscessus. Our data further suggested that M. salmoniphilum separates into three branches named group I, II and III with the M. salmoniphilum type strain belonging to group II. Among predicted virulence factors, the presence of phospholipase C (plcC), which is a major virulence factor that makes M. abscessus highly cytotoxic to mouse macrophages, and that M. franklinii originally was isolated from infected humans make it plausible that the outbreak in the animal facility was caused by a M. salmoniphilum-like strain. Interestingly, M. salmoniphilum-like was isolated from tap water suggesting that it can be present in the environment. Moreover, we predicted the presence of mutational hotspots in the M. salmoniphilum isolates and 26% of these hotspots overlap with genes categorized as having roles in virulence, disease and defense. We also provide data about key genes involved in transcription and translation such as sigma factor, ribosomal protein and tRNA genes.

Place, publisher, year, edition, pages
2019. Vol. 9, article id 4603
National Category
Microbiology in the medical area
Identifiers
URN: urn:nbn:se:uu:diva-380444DOI: 10.1038/s41598-019-40922-xISI: 000461159600080PubMedID: 30872669OAI: oai:DiVA.org:uu-380444DiVA, id: diva2:1300192
Funder
Swedish Research CouncilSwedish Research Council FormasSwedish Research CouncilAvailable from: 2019-03-28 Created: 2019-03-28 Last updated: 2019-03-28Bibliographically approved

Open Access in DiVA

fulltext(10026 kB)9 downloads
File information
File name FULLTEXT01.pdfFile size 10026 kBChecksum SHA-512
6088aa2ed6acf255e64033e35d1f648dadc78f22bf7b5fee7b2e35ab2b7a4d12f95f59a6bda55e0ebfc9ca4c89dd8a3722dab9d4b5a2909217e287c7015f5fcc
Type fulltextMimetype application/pdf

Other links

Publisher's full textPubMed

Authority records BETA

Behra, Phani Rama KrishnaDas, SarbashisPettersson, B. M. FredrikKirsebom, Leif A

Search in DiVA

By author/editor
Behra, Phani Rama KrishnaDas, SarbashisPettersson, B. M. FredrikKirsebom, Leif A
By organisation
Molecular BiologyDepartment of Neuroscience
In the same journal
Scientific Reports
Microbiology in the medical area

Search outside of DiVA

GoogleGoogle Scholar
Total: 9 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

doi
pubmed
urn-nbn

Altmetric score

doi
pubmed
urn-nbn
Total: 29 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf