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Detection of celery (Apium graveolens) in food with Real-Time PCR
Uppsala University, Medicinska vetenskapsområdet, Faculty of Medicine, Department of Medical Biochemistry and Microbiology.
2006 (English)Independent thesis Basic level (degree of Bachelor), 10 credits / 15 HE creditsStudent thesis
Abstract [en]

Directive EC 2003/89/EC of the European Parliament and of the Council states that certain

ingredients and products derived there of known to cause allergen reactions must always be

declared. Furthermore labelling is mandatory irrespective of the amount included. The National

Food Administration therefore needs methods for monitoring the presence of allergens in food.

Methods already exist for most of the allergens on the EU-list, but an operational method for

celery (Apium graveolens) is missing.

A specific DNA-method was developed, based on TaqMan Real-Time PCR with the celery

mannitol dehydrogenase gene as target sequence. The analysis was started with homogenisation

of the sample followed by extraction of DNA. The Real-Time PCR method was shown to be

specific for celery, producing a 113 bp fragment with two celery varieties and negative results

with other closely selected species commonly present together with celery in food products (12

samples). The detection limit was 2-20 pg DNA, which corresponds to 1-7 haploid genome

copies. When evaluated with model samples of celery in meat, a detection limit of less than

0,01 % was determined. When used to analyse food products from the market, six out of seven

products declared to contain celery were correctly identified as positive.

Place, publisher, year, edition, pages
Uppsala: Universitetsbiblioteket , 2006. , p. 14
Keywords [en]
Keywords: celery, allergen, DNA-method, RealTime-PCR, mannitol dehydrogenase
National Category
Biochemistry and Molecular Biology
Identifiers
URN: urn:nbn:se:uu:diva-7130OAI: oai:DiVA.org:uu-7130DiVA, id: diva2:131027
Uppsok
fysik/kemi/matematik
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Examiners
Available from: 2006-08-29 Created: 2006-08-29

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CiteExportLink to record
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Citation style
  • apa
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