uu.seUppsala University Publications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Proton MR spectroscopy of human pancreas allografts
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Surgical Sciences, Radiology.ORCID iD: 0000-0003-2448-9245
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Surgical Sciences, Radiology.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Immunology, Genetics and Pathology, Clinical Immunology.ORCID iD: 0000-0002-8524-9547
2019 (English)In: Magnetic Resonance Materials in Physics, Biology and Medicine, ISSN 0968-5243, E-ISSN 1352-8661, Vol. 32, no 4, p. 511-517Article in journal (Refereed) Published
Abstract [en]

OBJECTIVE: To estimate pancreas graft relaxation times and concentrations of total fat, and the intracellular lipids of non-adipose pancreatic cells (NAPC) using proton (1H) magnetic resonance spectroscopy (MRS) during cold preservation.

MATERIALS AND METHODS: Grafts from 11 human donors were investigated. Each pancreas was perfused in situ with histidine-tryptophan-ketoglutarate (HTK) or with University of Wisconsin solution and placed into a transport container. Temperature of the grafts was maintained at 4 ± 2 °C during transport to our hospital and MR scanning. A 1.5 T clinical scanner was used for the measurements. Single-voxel PRESS spectra were acquired using transmit-receiver head coil.

RESULTS: Relaxation times were measured for lipid (-CH2-)n (T1, 287 ± 60 ms; T2, 27 ± 4 ms), and tissue water (T1, 670 ± 69 ms; T2, 77 ± 17 ms). Average total fat, and intracellular lipids of NAPC concentrations were 79.2 ± 100.8 (range 2.4-304.4), and 2.9 ± 1.2 mmol/kg ww, respectively.

CONCLUSION: We have shown that 1H-MRS is a useful tool for the estimation of pancreas graft lipid concentrations. Total pancreatic fat and especially content of intracellular lipids of NAPC are valuable measures for inspection of graft quality prior to transplantation or islet of Langerhans isolation.

Place, publisher, year, edition, pages
2019. Vol. 32, no 4, p. 511-517
Keywords [en]
1H-MRS, Intracellular fat, Pancreas graft, Relaxation times, Total fat
National Category
Radiology, Nuclear Medicine and Medical Imaging
Identifiers
URN: urn:nbn:se:uu:diva-382935DOI: 10.1007/s10334-019-00740-8ISI: 000476510700009PubMedID: 30937576OAI: oai:DiVA.org:uu-382935DiVA, id: diva2:1314034
Funder
Swedish Research Council, VR K2013-64X-08268-26-3Swedish Research Council, 921-2014-7054Swedish Research Council, K2015-54X-12219-19-4Ernfors FoundationSwedish Child Diabetes FoundationSwedish Diabetes AssociationEXODIAB - Excellence of Diabetes Research in SwedenAvailable from: 2019-05-07 Created: 2019-05-07 Last updated: 2019-09-20Bibliographically approved

Open Access in DiVA

fulltext(717 kB)55 downloads
File information
File name FULLTEXT01.pdfFile size 717 kBChecksum SHA-512
f8e892f1aea498eeacaf9769910babd9c5c8acb8de6fdbdfe0d52ef56fcc534e1cb5e447623f8c8a0dc9f630c16d369559361f093cc00e13844853d324a56145
Type fulltextMimetype application/pdf

Other links

Publisher's full textPubMed

Authority records BETA

Weis, JanAhlström, HåkanKorsgren, Olle

Search in DiVA

By author/editor
Weis, JanAhlström, HåkanKorsgren, Olle
By organisation
RadiologyClinical Immunology
In the same journal
Magnetic Resonance Materials in Physics, Biology and Medicine
Radiology, Nuclear Medicine and Medical Imaging

Search outside of DiVA

GoogleGoogle Scholar
Total: 55 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

doi
pubmed
urn-nbn

Altmetric score

doi
pubmed
urn-nbn
Total: 82 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf