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A multiplex fluorescence microsphere immunoassay for increased understanding of Rift Valley fever immune responses in ruminants in Kenya
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Biochemistry and Microbiology. International Livestock Research Institute, Nairobi, Kenya;Swedish University of Agricultural Research, Uppsala, Sweden.ORCID iD: 0000-0002-1175-0398
Department of Biomedical Sciences, Colorado State University, Fort Collins, CO, USA;Department of Diagnostic Medicine and Pathobiology, College of Veterinary Medicine, Kansas State University, Manhattan, KS, USA.
Department of Diagnostic Medicine and Pathobiology, College of Veterinary Medicine, Kansas State University, Manhattan, KS, USA.
International Livestock Research Institute, Nairobi, Kenya.
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2019 (English)In: Journal of Virological Methods, ISSN 0166-0934, E-ISSN 1879-0984, Vol. 269, p. 70-76Article in journal (Refereed) Published
Abstract [en]

Rift Valley fever virus (RVFV) is an important mosquito-borne pathogen with devastating impacts on agriculture and public health. With outbreaks being reported beyond the continent of Africa to the Middle East, there is great concern that RVFV will continue to spread to non-endemic areas such as the Americas and Europe. There is a need for safe and high throughput serological assays for rapid detection of RVFV during outbreaks and for surveillance. We evaluated a multiplexing fluorescence microsphere immunoassay (FMIA) for the detection of IgG and IgM antibodies in ruminant sera against the RVFV nucleocapsid Np, glycoprotein Gn, and non-structural protein NSs. Sheep and cattle sera from a region in Kenya with previous outbreaks were tested by FMIA and two commercially available competitive ELISAs (BDSL and IDvet). Our results revealed strong detection of RVFV antibodies against the Np, Gn and NSs antigen targets. Additionally, testing of samples with FMIA Np and Gn had 100% agreement with the IDvet ELISA. The targets developed in the FMIA assay provided a basis for a larger ruminant disease panel that can simultaneously screen several abortive and zoonotic pathogens.

Place, publisher, year, edition, pages
Elsevier, 2019. Vol. 269, p. 70-76
Keywords [en]
Arbovirus, DIVA test, Seroconversion, Serology, Vector-borne disease
National Category
Veterinary Science
Identifiers
URN: urn:nbn:se:uu:diva-384636DOI: 10.1016/j.jviromet.2019.04.011ISI: 000469890400012PubMedID: 30974177OAI: oai:DiVA.org:uu-384636DiVA, id: diva2:1321163
Funder
Swedish Research CouncilAvailable from: 2019-06-07 Created: 2019-06-07 Last updated: 2019-06-26Bibliographically approved

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Lindahl, Johanna

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