A sensitive method detecting trace levels of levonorgestrel using LC-HRMS.Vise andre og tillknytning
2019 (engelsk)Inngår i: Contraception, ISSN 0010-7824, E-ISSN 1879-0518, Vol. 100, nr 3, s. 247-249Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]
OBJECTIVE: To develop a high resolution mass spectrometry (HRMS) method to quantify levonorgestrel (LNG) in serum.
STUDY DESIGN: Levonorgestrel was extracted using solid phase extraction and measured using liquid chromatography (LC) HRMS.
RESULTS: Low limit of quantification (LLOQ) was 25pg/mL and low limit of detection (LLOD) was 12.5pg/mL. Precision and accuracy bias were<10%. LNG in serum samples from Mirena® users ranged between 37 to 219pg/mL (n=12). In eight out of 22 patients with suspected intrauterine device (IUD) expulsion LNG was detected (26 to 1272pg/mL).
CONCLUSION: A sensitive, fast and simple LC-HRMS method was developed to detect trace levels of LNG.
sted, utgiver, år, opplag, sider
2019. Vol. 100, nr 3, s. 247-249
Emneord [en]
High resolution mass spectrometry, Intrauterine device, Liquid chromatography, Lower limit of quantification, Solid phase extraction
HSV kategori
Identifikatorer
URN: urn:nbn:se:uu:diva-388593DOI: 10.1016/j.contraception.2019.06.001ISI: 000483452100015PubMedID: 31216423OAI: oai:DiVA.org:uu-388593DiVA, id: diva2:1334174
2019-07-022019-07-022022-01-29bibliografisk kontrollert