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The effect of Wolbachia on gene expression in Drosophila paulistorum and its implications for symbiont-induced host speciation
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Cell and Molecular Biology, Molecular Evolution.
Med Univ Vienna, Ctr Anat & Cell Biol, Lab Genome Dynam, Deparment Cell & Dev Biol, Schwarzspanierstr 17, A-1090 Vienna, Austria;Yale Univ, Dept Epidemiol Microbial Dis, 60 Coll St, New Haven, CT 06510 USA.
Med Univ Vienna, Ctr Anat & Cell Biol, Lab Genome Dynam, Deparment Cell & Dev Biol, Schwarzspanierstr 17, A-1090 Vienna, Austria.
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Cell and Molecular Biology, Molecular Evolution.ORCID iD: 0000-0002-5874-7153
2019 (English)In: BMC Genomics, ISSN 1471-2164, E-ISSN 1471-2164, Vol. 20, article id 465Article in journal (Refereed) Published
Abstract [en]

Background: The Neotropical fruit fly Drosophila paulistorum (Diptera: Drosophilidae) is a species complex in statu nascendi comprising six reproductively isolated semispecies, each harboring mutualistic Wolbachia strains. Although wild type flies of each semispecies are isolated from the others by both pre- and postmating incompatibilities, mating between semispecies and successful offspring development can be achieved once flies are treated with antibiotics to reduce Wolbachia titer. Here we use RNA-seq to study the impact of Wolbachia on D. paulistorum and investigate the hypothesis that the symbiont may play a role in host speciation. For that goal, we analyze samples of heads and abdomens of both sexes of the Amazonian, Centro American and Orinocan semispecies of D. paulistorum.

Results: We identify between 175 and 1192 differentially expressed genes associated with a variety of biological processes that respond either globally or according to tissue, sex or condition in the three semispecies. Some of the functions associated with differentially expressed genes are known to be affected by Wolbachia in other species, such as metabolism and immunity, whereas others represent putative novel phenotypes involving muscular functions, pheromone signaling, and visual perception.

Conclusions: Our results show that Wolbachia affect a large number of biological functions in D. paulistorum, particularly when present in high titer. We suggest that the significant metabolic impact of the infection on the host may cause several of the other putative and observed phenotypes. We also speculate that the observed differential expression of genes associated with chemical communication and reproduction may be associated with the emergence of pre- and postmating barriers between semispecies, which supports a role for Wolbachia in the speciation of D. paulistorum.

Place, publisher, year, edition, pages
2019. Vol. 20, article id 465
Keywords [en]
Speciation, symbiosis, Wolbachia, transcriptome, Drosophila paulistorum, host-symbiont interactions
National Category
Genetics Evolutionary Biology
Identifiers
URN: urn:nbn:se:uu:diva-389597DOI: 10.1186/s12864-019-5816-9ISI: 000470715200001PubMedID: 31174466OAI: oai:DiVA.org:uu-389597DiVA, id: diva2:1338709
Funder
Swedish Research Council, 2014-4353Available from: 2019-07-24 Created: 2019-07-24 Last updated: 2019-07-24Bibliographically approved

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Baiao, Guilherme CostaKlasson, Lisa

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