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Single-Molecule Kinetics in Living Cells
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Cell and Molecular Biology, Molecular Systems Biology.ORCID iD: 0000-0001-5522-1810
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Biochemistry and Microbiology. Uppsala University, Science for Life Laboratory, SciLifeLab.
2019 (English)In: Annual Review of Biochemistry, ISSN 0066-4154, E-ISSN 1545-4509, Vol. 88, p. 635-659Article, review/survey (Refereed) Published
Abstract [en]

In the past decades, advances in microscopy have made it possible to study the dynamics of individual biomolecules in vitro and resolve intramolecular kinetics that would otherwise be hidden in ensemble averages. More recently, single-molecule methods have been used to image, localize, and track individually labeled macromolecules in the cytoplasm of living cells, allowing investigations of intermolecular kinetics under physiologically relevant conditions. In this review, we illuminate the particular advantages of single-molecule techniques when studying kinetics in living cells and discuss solutions to specific challenges associated with these methods.

Place, publisher, year, edition, pages
ANNUAL REVIEWS, 2019. Vol. 88, p. 635-659
Keywords [en]
single-molecule microscopy, single-molecule kinetics, fluorescence labeling, single-molecule tracking, spot localization, simulated microscopy
National Category
Biochemistry and Molecular Biology
Identifiers
URN: urn:nbn:se:uu:diva-390235DOI: 10.1146/annurev-biochem-013118-110801ISI: 000472818600025PubMedID: 30359080ISBN: 978-0-8243-0888-9 (print)OAI: oai:DiVA.org:uu-390235DiVA, id: diva2:1341427
Available from: 2019-08-08 Created: 2019-08-08 Last updated: 2019-08-08Bibliographically approved

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Elf, JohanBarkefors, Irmeli

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Molecular Systems BiologyDepartment of Medical Biochemistry and MicrobiologyScience for Life Laboratory, SciLifeLab
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