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Giardia intestinalis cystatin is a potent inhibitor of papain, parasite cysteine proteases and, to a lesser extent, human cathepsin B
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Cell and Molecular Biology, Microbiology.
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Cell and Molecular Biology, Microbiology.ORCID iD: 0000-0002-7392-1746
Robert Koch Inst, Dept Mycot & Parasit Agents & Mycobacteria FG16, Seestr 10, D-13353 Berlin, Germany.
2019 (English)In: FEBS Letters, ISSN 0014-5793, E-ISSN 1873-3468, Vol. 593, no 12, p. 1313-1325Article in journal (Refereed) Published
Abstract [en]

Cystatins are important regulators of papain-like cysteine proteases. In the protozoan parasite Giardia intestinalis, papain-like cysteine proteases play an essential role in the parasite's biology and pathogenicity. Here, we characterized a cysteine protease inhibitor of G. intestinalis that belongs to type-I-cystatins. The parasite cystatin is shown to be a strong inhibitor of papain (K-i approximate to 0.3 nm) and three parasite cysteine proteases (CP14019, CP16160 and CP16779, K-i approximate to 0.9-5.8 nm), but a weaker inhibitor of human cathepsin B (K-i approximate to 79.9 nm). The protein localizes mainly in the cytoplasm. Together, these data suggest that cystatin of G. intestinalis plays a role in the regulation of cysteine protease activities in the parasite and, possibly, in the interaction with the host.

Place, publisher, year, edition, pages
John Wiley & Sons, 2019. Vol. 593, no 12, p. 1313-1325
Keywords [en]
cystatin, cysteine protease inhibitor, cysteine proteases, Giardia intestinalis, protozoa
National Category
Biochemistry and Molecular Biology
Identifiers
URN: urn:nbn:se:uu:diva-390594DOI: 10.1002/1873-3468.13433ISI: 000472673700005PubMedID: 31077354OAI: oai:DiVA.org:uu-390594DiVA, id: diva2:1342360
Funder
Swedish Research Council, 2017-02918Available from: 2019-08-13 Created: 2019-08-13 Last updated: 2019-08-13Bibliographically approved

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Liu, JingyiSvärd, Staffan

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