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Peptide-Loaded Cubosomes Functioning as an Antimicrobial Unit against Escherichia coil
RISE Res Inst Sweden, S-50115 Boras, Sweden;Chalmers Univ Technol, Dept Chem & Chem Engn, S-41296 Gothenburg, Sweden.
Univ Copenhagen, Dept Pharm, DK-2100 Copenhagen, Denmark.ORCID iD: 0000-0002-0296-0369
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Pharmacy, Department of Pharmacy.ORCID iD: 0000-0001-5626-3959
Rutherford Appleton Lab, Didcot OX11 0DE, Oxon, England.
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2019 (English)In: ACS Applied Materials and Interfaces, ISSN 1944-8244, E-ISSN 1944-8252, Vol. 11, no 24, p. 21314-21322Article in journal (Refereed) Published
Abstract [en]

Dispersions of cubic liquid crystalline phases, also known as cubosomes, have shown great promise as delivery vehicles for a wide range of medicines. Due to their ordered structure, comprising alternating hydrophilic and hydrophobic domains, cubosomes possess unique delivery properties and compatibility with both water-soluble and-insoluble drugs. However, the drug delivery mechanism and cubosome interaction with human cells and bacteria are still poorly understood. Herein, we reveal how cubosomes loaded with the human cathelicidin antimicrobial peptide LL-37, a system with high bacteria-killing effect, interact with the bacterial membrane and provide new insights into the eradication mechanism. Combining the advanced experimental techniques neutron reflectivity and quartz crystal microbalance with dissipation monitoring, a mechanistic drug delivery model for LL-37-loaded cubosomes on bacterial mimicking bilayers was constructed. Moreover, the cubosome interaction with Escherichia coli was directly visualized using super-resolution laser scanning microscopy and cryogenic electron tomography. We could conclude that cubosomes loaded with LL-37 adsorbed and distorted bacterial membranes, providing evidence that the peptide-loaded cubosomes function as an antimicrobial unit.

Place, publisher, year, edition, pages
American Chemical Society (ACS), 2019. Vol. 11, no 24, p. 21314-21322
Keywords [en]
cubosome, antimicrobial peptide, LL-37, bacteria, membrane
National Category
Pharmacology and Toxicology
Identifiers
URN: urn:nbn:se:uu:diva-390683DOI: 10.1021/acsami.9b01826ISI: 000472683300004PubMedID: 31120236OAI: oai:DiVA.org:uu-390683DiVA, id: diva2:1342896
Funder
EU, FP7, Seventh Framework Programme, 604182Available from: 2019-08-14 Created: 2019-08-14 Last updated: 2019-08-14Bibliographically approved

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Nordström, Randi

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