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A portable epigenetic switch for bistable gene expression in bacteria
Univ Seville, Fac Biol, Dept Genet, Apartado 1095, E-41080 Seville, Spain.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Biochemistry and Microbiology.
Univ Seville, Fac Biol, Dept Genet, Apartado 1095, E-41080 Seville, Spain.
Univ Seville, Fac Biol, Dept Genet, Apartado 1095, E-41080 Seville, Spain;UB, UAB, IRTA, Ctr Res Agr Genom,CSIC, Campus UAB, Barcelona 08193, Spain.
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2019 (English)In: Scientific Reports, ISSN 2045-2322, E-ISSN 2045-2322, Vol. 9, article id 11261Article in journal (Refereed) Published
Abstract [en]

We describe a portable epigenetic switch based on opvAB, a Salmonella enterica operon that undergoes bistable expression under DNA methylation control. A DNA fragment containing the opvAB promoter and the opvAB upstream regulatory region confers bistability to heterologous genes, yielding OFF and ON subpopulations. Bistable expression under opvAB control is reproducible in Escherichia coli, showing that the opvAB switch can be functional in a heterologous host. Subpopulations of different sizes can be produced at will using engineered opvAB variants. Controlled formation of antibiotic-resistant and antibiotic-susceptible subpopulations may allow use of the opvAB switch in the study of bacterial heteroresistance to antibiotics.

Place, publisher, year, edition, pages
NATURE PUBLISHING GROUP , 2019. Vol. 9, article id 11261
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Biochemistry and Molecular Biology
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URN: urn:nbn:se:uu:diva-392133DOI: 10.1038/s41598-019-47650-2ISI: 000478575000048PubMedID: 31375711OAI: oai:DiVA.org:uu-392133DiVA, id: diva2:1347864
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Swedish Research Council, 2017-01527Available from: 2019-09-02 Created: 2019-09-02 Last updated: 2019-09-02Bibliographically approved

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Nicoloff, HervéAndersson, Dan I

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