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4 '-Guanidinium-modified siRNA: a molecular tool to control RNAi activity through RISC priming and selective antisense strand loading
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - Ångström, Polymer Chemistry.ORCID iD: 0000-0002-7256-0758
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - Ångström, Polymer Chemistry.
Bose Inst, Dept Biophys, Biomol NMR & Drug Design Lab, P-1-12 CIT Scheme 7M, Kolkata, India.
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - Ångström, Polymer Chemistry.
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2019 (English)In: Chemical Communications, ISSN 1359-7345, E-ISSN 1364-548X, Vol. 55, no 62, p. 9112-9115Article in journal (Refereed) Published
Abstract [en]

We designed novel 4 '-C-guanidinocarbohydrazidomethyl-5-methyl uridine (GMU) modified small interfering RNA (siRNA) and evaluated its biophysical and biochemical properties. Incorporation of GMU units significantly increased the thermodynamic stability as well as the enzymatic stability against nucleases in human serum. A gene silencing experiment indicated that GMU modfied siRNA (siRNA6) resulted in approximate to 4.9-fold more efficient knockdown than unmodified siRNA.

Place, publisher, year, edition, pages
ROYAL SOC CHEMISTRY , 2019. Vol. 55, no 62, p. 9112-9115
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Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)
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URN: urn:nbn:se:uu:diva-392130DOI: 10.1039/c9cc04141aISI: 000477960000008PubMedID: 31298670OAI: oai:DiVA.org:uu-392130DiVA, id: diva2:1348151
Note

Correction in: CHEMICAL COMMUNICATIONS, Volume: 55, Issue: 67, Pages: 10028-10028, DOI: 10.1039/c9cc90352f

Available from: 2019-09-03 Created: 2019-09-03 Last updated: 2019-09-25Bibliographically approved

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Nawale, Ganesh N.Kadekar, SandeepVarghese, Oommen P.

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