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A turn-on fluorescent GFP chromophore analog for highly selective and efficient detection of H2S in aqueous and in living cells
Univ Mumbai, Dept Chem, Mumbai 400098, Maharashtra, India.
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - Ångström, Polymer Chemistry.ORCID iD: 0000-0002-7256-0758
Indian Inst Technol, Dept Chem, Mumbai 400076, Maharashtra, India.
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - Ångström, Polymer Chemistry.ORCID iD: 0000-0001-8872-9928
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2019 (English)In: Sensors and actuators. B, Chemical, ISSN 0925-4005, E-ISSN 1873-3077, Vol. 298, article id 126875Article in journal (Refereed) Published
Abstract [en]

Hydrogen sulphide is a gaseous neurotransmitter responsible for neuronal function and controls vast range of physiological functions. Herein, we report the synthesis and evaluation of novel Green Fluorescent Protein (GFP) chromophore analog, acryloyl-4-(p-hydroxybenzylidene)-5-imidazolidinone (AHBI) for turn-on fluorescent detection of H2S over wide range of anions and various biologically important competitive thiols. AHBI probe exhibited high selectivity and sensitivity, high fluorescence stability, large stokes shift and lower detection limit (15.85 ppb) for H2S in complete water medium. Cell imaging studies in human colon cancer cells (HCT116) and normal human dermal fibroblasts (HDF) confirmed the compatibility and versatility of AHBI probe at micromolar level. Overall, we believe the AHBI, as an optical probe will be useful to investigate the role of H2S in various physiological processes, regulation of cancer cell growth, and in pathogenic events.

Place, publisher, year, edition, pages
ELSEVIER SCIENCE SA , 2019. Vol. 298, article id 126875
Keywords [en]
Green fluorescent protein, Chemosensor, H2S detection, Cysteine, Live cell imaging
National Category
Biochemistry and Molecular Biology
Identifiers
URN: urn:nbn:se:uu:diva-394184DOI: 10.1016/j.snb.2019.126875ISI: 000483419800053OAI: oai:DiVA.org:uu-394184DiVA, id: diva2:1359536
Available from: 2019-10-09 Created: 2019-10-09 Last updated: 2019-10-09Bibliographically approved

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Nawale, Ganesh N.Varghese, Oommen P.

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