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A simplified and sensitive immunoprecipitation mass spectrometry protocol for the analysis of amyloid-beta peptides in brain tissue
Charite Univ Med Berlin, Dept Neuropathol AG Heppner, Campus Mitte,Charitapl 1, DE-10117 Berlin, Germany;Univ Med Gottingen, Dept Psychiat & Psychotherapy, von Siebold Str 5, DE-37075 Gottingen, Germany.
Charite Univ Med Berlin, Dept Neuropathol AG Heppner, Campus Mitte,Charitapl 1, DE-10117 Berlin, Germany;Univ Med Gottingen, Dept Psychiat & Psychotherapy, von Siebold Str 5, DE-37075 Gottingen, Germany.
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - BMC, Analytical Chemistry.ORCID iD: 0000-0002-9510-3816
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - BMC, Analytical Chemistry.
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2019 (English)In: CLINICAL MASS SPECTROMETRY, ISSN 2376-9998, Vol. 14, p. 83-88Article in journal (Refereed) Published
Abstract [en]

In the field of Alzheimer's disease, there is an urgent need for novel analytical tools to identify disease-specific biomarkers and to evaluate therapeutics. Preclinical trials commonly employ amyloid beta (A beta) peptide signatures as a read-out. In this paper, we report a simplified and detailed protocol for robust immunoprecipitation of A beta in brain tissue prior to mass spectrometric detection exemplified by a study using transgenic mice. The established method employed murine monoclonal and rabbit polyclonal antibodies and was capable of yielding well-reproducible peaks of high intensity with low background signal intensities corresponding to various A beta forms.

Place, publisher, year, edition, pages
ELSEVIER , 2019. Vol. 14, p. 83-88
Keywords [en]
MALDI-TOF MS, Amyloid beta peptides, Brain, Immunoprecipitation
National Category
Biochemistry and Molecular Biology
Identifiers
URN: urn:nbn:se:uu:diva-398015DOI: 10.1016/j.clinms.2019.07.001ISI: 000496422100004OAI: oai:DiVA.org:uu-398015DiVA, id: diva2:1375276
Funder
Swedish Foundation for Strategic Research , SB16-0039Swedish Research Council, 621-2011-4423Swedish Research Council, 2015-4870Magnus Bergvall Foundation, 2018-01726Available from: 2019-12-04 Created: 2019-12-04 Last updated: 2019-12-04Bibliographically approved

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Lind, SaraShevchenko, GannaBergquist, Jonas

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