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Toxicity study on Clinacanthus nutans leaf hexane fraction using Danio rerio embryos
Int Islamic Univ Malaysia, Dept Pharmaceut Chem, Kulliyyah Pharm, Pharmacognosy Res Grp, Kuantan, Pahang Darul Ma, Malaysia;Univ Kuala Lumpur, Fac Pharm & Hlth Sci, Perak Darul Ridzuan, Royal Coll Med Perak, Ipoh 30450, Perak Darul Rid, Malaysia.
Int Islamic Univ Malaysia, Dept Pharmaceut Chem, Kulliyyah Pharm, Pharmacognosy Res Grp, Kuantan, Pahang Darul Ma, Malaysia;Univ Malaysia, Int Islamic, Kulliyyah Sci, Cent Res & Anim Facil, Kuantan 25200, Pahang Darul Ma, Malaysia;Airlangga Univ, Fac Pharm, Surabaya 60155, Indonesia.
Int Islamic Univ Malaysia, Dept Pharmaceut Chem, Kulliyyah Pharm, Pharmacognosy Res Grp, Kuantan, Pahang Darul Ma, Malaysia.
Int Islamic Univ Malaysia, Dept Pharmaceut Chem, Kulliyyah Pharm, Pharmacognosy Res Grp, Kuantan, Pahang Darul Ma, Malaysia.
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2019 (English)In: Toxicology reports, ISSN 1972-6325, E-ISSN 2214-7500, Vol. 6, p. 1148-1154Article in journal (Refereed) Published
Abstract [en]

Clinacanthus nutans, an herbal shrub belonging to the Acanthaceae family, is traditionally used as a functional food to treat various ailments in Malaysia and Indonesia. Although the polar fraction of this plant shows nontoxic effect, the toxicity of the non-polar extract is not reported so far. The present study aimed to assess the toxic effect and determine the lethal concentration of this non-polar fraction using zebrafish embryos. The n-hexane fraction was partitioned from the crude extract of C. nutans obtained using 80% methanolic solution. After spawning of the adult male and female zebrafish, the eggs were collected, transferred into a 96-well plate and incubated with the n-hexane fraction at concentrations of 15.63 mu g/ml, 31.25 mu g/ml, 62.5 mu g/ml, 125 mu g/ml, 250 mu g/ml and 500 mu g/ml in 2% DMSO. The survival and sublethal endpoint were assessed, the mortality and hatchability rates were calculated based on microscopic observation, while the heartbeat rate was measured using DanioScope software. The median lethal concentration (LC50) of the C. nutans n-hexane fraction, which was determined using probit analysis, was calculated to be 75.49 mu g/mL, which is harmful. Moreover, gas chromatography-mass spectrometry (GC-MS) analysis revealed the presence of palmitic acid, phytol, hexadecanoic acid, 1-monopalmitin, stigmast-5-ene, pentadecanoic acid, heptadecanoic acid, 1-linolenoylglycerol and stigmasterol in the n-hexane fraction.

Place, publisher, year, edition, pages
ELSEVIER , 2019. Vol. 6, p. 1148-1154
Keywords [en]
Clinacanthus nutans, Danio rerio, DanioScope, Median lethal concentration, Teratogenicity, Probit analysis
National Category
Environmental Sciences
Identifiers
URN: urn:nbn:se:uu:diva-400826DOI: 10.1016/j.toxrep.2019.10.020ISI: 000501571200138OAI: oai:DiVA.org:uu-400826DiVA, id: diva2:1384926
Funder
Swedish Research Council, 2016-05908Available from: 2020-01-13 Created: 2020-01-13 Last updated: 2020-01-13Bibliographically approved

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El-Seedi, Hesham

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